华东理工大学学报：社会科学版
華東理工大學學報：社會科學版
화동리공대학학보：사회과학판
SOCIAL SCIENCES JOURNAL OF ECUST
2007年
5期
~
,共null页
p66shc基因 荧光素酶 药物筛选
p66shc基因 熒光素酶 藥物篩選
p66shc기인 형광소매 약물사선
p66~（shc） gene; luciferase; drug screening;
将p66shc基因的启动子序列克隆到含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,然后将重组质粒pGL3B-neo-p66shc转染入人的肺癌细胞A549中,通过单集落分离法得到含有重组质粒的稳定细胞系A549/p66shc。利用这个以报告基因为基础的药物普筛系统,筛选了数百种中药提取物及单体化合物,发现抑制率达到50%以上的2种中药提取物和8种单体化合物。
將p66shc基因的啟動子序列剋隆到含有熒光素酶報告基因和新黴素抗性基因的pGL3B-neo載體中,然後將重組質粒pGL3B-neo-p66shc轉染入人的肺癌細胞A549中,通過單集落分離法得到含有重組質粒的穩定細胞繫A549/p66shc。利用這箇以報告基因為基礎的藥物普篩繫統,篩選瞭數百種中藥提取物及單體化閤物,髮現抑製率達到50%以上的2種中藥提取物和8種單體化閤物。
장p66shc기인적계동자서렬극륭도함유형광소매보고기인화신매소항성기인적pGL3B-neo재체중,연후장중조질립pGL3B-neo-p66shc전염입인적폐암세포A549중,통과단집락분리법득도함유중조질립적은정세포계A549/p66shc。이용저개이보고기인위기출적약물보사계통,사선료수백충중약제취물급단체화합물,발현억제솔체도50%이상적2충중약제취물화8충단체화합물。
The promoter of p66~(shc) gene was cloned into the pGL3B-neo vector containing the luciferase gene and neomycin resistance gene,and the recombinant vector pGL3B-neo-p66~(shc) was subsequently transfected into A549 cells,and then the stable cell line A549/p66~(shc) was obtained through single colony iso-(lation.) Using this reporter gene-based screening system,several hundred samples were screened and ten of them were found to meet demands.
