CAJ | 학술논문

在枯草芽孢杆菌突变株D-756发酵生产D-核糖过程中,采用葡萄糖和葡萄糖酸钙混合发酵,葡萄糖酸作为pH调节剂,能促进菌体的生长,显著地提高D-核糖的产量。经酶活测定,发现流加葡萄糖酸能提高单位发酵液中葡萄糖酸激酶的酶活。在5 L发酵罐中,利用葡萄糖酸维持发酵pH值在7.2,培养72 h后产核糖76.8 g/L。
재고초아포간균돌변주D-756발효생산D-핵당과정중,채용포도당화포도당산개혼합발효,포도당산작위pH조절제,능촉진균체적생장,현저지제고D-핵당적산량。경매활측정,발현류가포도당산능제고단위발효액중포도당산격매적매활。재5 L발효관중,이용포도당산유지발효pH치재7.2,배양72 h후산핵당76.8 g/L。
During the fermentation of D-ribose with glucose and calcium gluconate mixture by Bacillus subtilis mutant D-756, the cell growth was improved and the yield of D-ribose was significantly increased through the feeding of gluconate as pH regulator.And the effect on the key enzymes involved in D-ribose production was studied.The activity of gluconate kinase in unit fermentation volume could be enhanced by addition of gluconate.In addition,the cells growth and D-ribose accumulation were not affected under weak ...