华东理工大学学报：社会科学版
華東理工大學學報：社會科學版
화동리공대학학보：사회과학판
SOCIAL SCIENCES JOURNAL OF ECUST
2005年
6期
~
,共null页
谌颉 储炬 庄英萍 张嗣良
諶頡 儲炬 莊英萍 張嗣良
심힐 저거 장영평 장사량
有机酸 阿维链霉菌 发酵过程 生物合成
有機痠 阿維鏈黴菌 髮酵過程 生物閤成
유궤산 아유련매균 발효과정 생물합성
organic acid; S.avermitilis; fermentation process; biosynthesis;
对S.averm itilis发酵过程菌体胞内与胞外的几种有机酸浓度进行跟踪测定。通过与产素关联发现低产批次与高产批次有机酸积累有明显不同。低产批次胞内丙酮酸浓度高达31.6 m g/L,此后降至5 m g/L以下,而胞外在80 h后都开始出现积累;乙酸、柠檬酸与琥珀酸一直都大量出现在胞外,如柠檬酸胞内胞外浓度比值初期低于0.1。这体现出生长期延长以致与产素期重叠过多造成产素偏低。
對S.averm itilis髮酵過程菌體胞內與胞外的幾種有機痠濃度進行跟蹤測定。通過與產素關聯髮現低產批次與高產批次有機痠積纍有明顯不同。低產批次胞內丙酮痠濃度高達31.6 m g/L,此後降至5 m g/L以下,而胞外在80 h後都開始齣現積纍;乙痠、檸檬痠與琥珀痠一直都大量齣現在胞外,如檸檬痠胞內胞外濃度比值初期低于0.1。這體現齣生長期延長以緻與產素期重疊過多造成產素偏低。
대S.averm itilis발효과정균체포내여포외적궤충유궤산농도진행근종측정。통과여산소관련발현저산비차여고산비차유궤산적루유명현불동。저산비차포내병동산농도고체31.6 m g/L,차후강지5 m g/L이하,이포외재80 h후도개시출현적루;을산、저몽산여호박산일직도대량출현재포외,여저몽산포내포외농도비치초기저우0.1。저체현출생장기연장이치여산소기중첩과다조성산소편저。
Intercellular and extracellular organic acid concentrations of industrial culture of S.avermitilis were analyzed in several typical batches of avermectin fermentation.Experimental results showed that organic acids accumulation in low production batches and high ones were totally different.In low production batch, intercellular pyruvic acid concentrations reached 31.6 mg/L at early stage of process,then declined to less than 5 mg/L,while excretion of pyruvic acid could be observed after 80 h of cultivation.G...
