CAJ | 학술논문

通过构建细胞沉降过程的理想化模型,提出了一种简易的细胞沉降速率测定方法。标准颗粒沉降实验结果验证了这种方法的准确性。利用该方法测定杂交瘤细胞的沉降速率,定量描述了杂交瘤活细胞与死细胞之间的沉降速率差异,活细胞的沉降速率(15.5mm/h)大约是死细胞(9.36mm/h)的1.67倍,约是细胞碎片(1.46mm/h)的10倍;把该方法用于不同种类不同培养时期的脐血造血细胞,发现它们具有不同的沉降速率。这些数据将为细胞连续灌注培养过程的细胞截留研究提供依据。
통과구건세포침강과정적이상화모형,제출료일충간역적세포침강속솔측정방법。표준과립침강실험결과험증료저충방법적준학성。이용해방법측정잡교류세포적침강속솔,정량묘술료잡교류활세포여사세포지간적침강속솔차이,활세포적침강속솔(15.5mm/h)대약시사세포(9.36mm/h)적1.67배,약시세포쇄편(1.46mm/h)적10배;파해방법용우불동충류불동배양시기적제혈조혈세포,발현타문구유불동적침강속솔。저사수거장위세포련속관주배양과정적세포절류연구제공의거。
Perfusion has been verified as one of efficient and economical choice for animal cell culture bioprocesses. Cell retention within the perfusion system determines the efficiency of the whole processes to a great extent. While the settling velocity of animal cells plays an important role in the design of the cell retention device and the estimation of settling operation, the measurement of this critical parameter suffers from the lack of simple and accurate method for a long time. In this work a novel method ...