CAJ | 학술논문

采用MutS表型的重组毕赤酵母生产血管生长抑制素,表达阶段流加甘油-甲醇混合碳源以提高菌体密度和血管生长抑制素的表达水平,菌体密度可达174g/L,约是表达阶段采用甲醇为单一碳源的发酵过程的3倍。菌体密度的提高导致表达阶段发酵液中铵离子浓度下降很快,当发酵液中的铵离子浓度低至40mmol/L时,影响了血管生长抑制素的表达。改变pH调节方式并在发酵后期添加25mmol/L(NH4)2SO4使发酵液中铵离子浓度维持在150mmol/L以上,血管生长抑制素的表达产量达到108mg/L。
채용MutS표형적중조필적효모생산혈관생장억제소,표체계단류가감유-갑순혼합탄원이제고균체밀도화혈관생장억제소적표체수평,균체밀도가체174g/L,약시표체계단채용갑순위단일탄원적발효과정적3배。균체밀도적제고도치표체계단발효액중안리자농도하강흔쾌,당발효액중적안리자농도저지40mmol/L시,영향료혈관생장억제소적표체。개변pH조절방식병재발효후기첨가25mmol/L(NH4)2SO4사발효액중안리자농도유지재150mmol/L이상,혈관생장억제소적표체산량체도108mg/L。
In fed-batch cultivation of recombinant Pichia pastoris (Mut~S phenotype), feeding with glycerol and methanol was conducted during the expression phase to enhance the cell growth and angiostatin expression. The cell density reached 174 g/L at the end of fermentation, which was about 3 fold of that obtained with methanol as the sole carbon source in the induction phase. High cell density resulted in a quick drop of ammonium concentration in the fermentation broth, and when the ammonium concentration was belo...