CAJ | 학술논문

通过两步溶液聚合法合成表面含有羧基的聚氨酯(PU),并用1-乙基-3-(二甲基丙胺)碳二亚胺(WSC)对聚氨酯表面羧基进行活化后,在4℃下柠檬酸钠缓冲液中与肝素上氨基反应得到表面共价键接枝的肝素化聚氨酯膜。通过甲苯胺蓝染色法、红外光谱法等对肝素化前后结构特征进行表征,用静态水接触角、体外抗凝血性测试其生物相容性。结果表明:在pH=5.0的肝素柠檬酸钠缓冲液中反应96 h,聚氨酯表面肝素接枝率最高,接枝量达到1.92μg/cm2;同时,共价接枝肝素后接触角从61°降低到44°,并且抗凝血性能得到明显的改善。
통과량보용액취합법합성표면함유최기적취안지(PU),병용1-을기-3-(이갑기병알)탄이아알(WSC)대취안지표면최기진행활화후,재4℃하저몽산납완충액중여간소상안기반응득도표면공개건접지적간소화취안지막。통과갑분알람염색법、홍외광보법등대간소화전후결구특정진행표정,용정태수접촉각、체외항응혈성측시기생물상용성。결과표명:재pH=5.0적간소저몽산납완충액중반응96 h,취안지표면간소접지솔최고,접지량체도1.92μg/cm2;동시,공개접지간소후접촉각종61°강저도44°,병차항응혈성능득도명현적개선。
Polyurethanes containing carboxyl group were synthesized by two-step solution polymeri-(zation.) Heparin was immobilized on the PU surface via reacting between carboxyl group and the amino group in the heparin by covalent immobilization technology in heparin sodium citrate buffer at 4 ℃.The(heparinized) polyurethanes were investigated by FT-IR,water contact angle and antithrombin tests.(Results)(show that) the highest heparin content of PU surface is in heparin sodium citrate buffer(pH=5.0) for 96 h.(The wa...