CAJ | 학술논문

实验旨在观察预热休克对一次性大负荷运动造成大鼠骨骼肌运动性损伤是否具有保护作用.实验采用大鼠热休克模型,用物理加热方法使大鼠体温升高到40.5℃～41.5℃,持续10～15 min,在室温下恢复24 h后进行一次性大负荷运动(速度为27 m/min,坡度0°,时间为90min).通过对比预热和室温组大鼠运动后即刻、24 h、48 h血清中的肌酸激酶(CK)、乳酸脱氢酶(LDH),来确定预热休克对大鼠运动所致骨骼肌损伤是否具有保护作用,以及是否能够加速骨骼肌运动性损伤的恢复.结果发现,预热组运动后即刻、24 h,血清中激酶和乳酸脱氢酶活性均比室温组低(P＜0.05);运动后48 h,预热组较室温组血清中肌酸激酶和乳酸脱氢酶活性略低,没有显著性差异,两组肌酸激酶值比室温基础组略高,也没有显著性差异;预热运动后48 h组乳酸脱氢酶与室温基础组相比略高,没有显著性差异;室温运动后48 h组乳酸脱氢酶较室温基础组明显偏高,具有显著性差异(P＜0.05).提示,预热休克对于一次性大负荷运动所致的大鼠骨骼肌运动性损伤具有保护作用,并且能够促进骨骼肌运动性损伤的恢复.
실험지재관찰예열휴극대일차성대부하운동조성대서골격기운동성손상시부구유보호작용.실험채용대서열휴극모형,용물리가열방법사대서체온승고도40.5℃～41.5℃,지속10～15 min,재실온하회복24 h후진행일차성대부하운동(속도위27 m/min,파도0°,시간위90min).통과대비예열화실온조대서운동후즉각、24 h、48 h혈청중적기산격매(CK)、유산탈경매(LDH),래학정예열휴극대대서운동소치골격기손상시부구유보호작용,이급시부능구가속골격기운동성손상적회복.결과발현,예열조운동후즉각、24 h,혈청중격매화유산탈경매활성균비실온조저(P＜0.05);운동후48 h,예열조교실온조혈청중기산격매화유산탈경매활성략저,몰유현저성차이,량조기산격매치비실온기출조략고,야몰유현저성차이;예열운동후48 h조유산탈경매여실온기출조상비략고,몰유현저성차이;실온운동후48 h조유산탈경매교실온기출조명현편고,구유현저성차이(P＜0.05).제시,예열휴극대우일차성대부하운동소치적대서골격기운동성손상구유보호작용,병차능구촉진골격기운동성손상적회복.
The main purpose was to investigate whether heat shock had protection on exercise injury of skeletal muscle for 1 maximal training load in rat. All rats of heat groups were subjected to whole-body hyperthermia at 40. 5℃-41. 5℃ for 10-15 minutes. Rats were allowed to recovery from whole-body hyperthermia for 24 hours. Skeletal muscle was obtained from rats at right now,24 hours and 48 hours after heat shock to verify expression of HSP70. The result showed that comparing with the heating group ,exercise protocols of plasma enzymes resulted in significantly increase immediately and 24 hours after running,while 48 hours after exercise ,all protocols returned to pre-exercise level except for that activity of LDH. The activity of plasma CK and LDH in heating group were significantly decreased comparing with normal temperature group. However, the activity of plasma CK and LDH has no significant difference between two groups 48 hours after exercise. It suggested that heat shock had protection on exercise injury of skeletal muscle for 1 maximal training load in rat and improved the recovery of exercise injury of muscle.