体育科学
體育科學
체육과학
China Sport Science
2005年
12期
61~64
,共null页
螺旋藻 运动性疲劳 基因表达谱 鼠 动物实验
螺鏇藻 運動性疲勞 基因錶達譜 鼠 動物實驗
라선조 운동성피로 기인표체보 서 동물실험
spirulina＇s complex prescriptions; available composition; exercise fatigue; gene expression profile ; rat ;animal experiment
目的:研究螺旋藻复方有效部位配方对慢性运动性疲劳大鼠脑组织基因表达谱的影响,探索螺旋藻复方有效部位配方抗疲劳的分子药理作用机制.方法:分别从慢性运动性疲劳模型组、螺旋藻复方有效部位配方治疗组SD大鼠的脑组织中抽提总RNA,Cy3、Cy5荧光标记,反转录分别合成cDNA探针后,与含有4096个大鼠基因的BiostarR-20S基因表达谱芯片杂交,Genepix 4000B扫描仪扫描,GenePixPro 3.0软件分析表达信号.结果:螺旋藻复方有效部位配方治疗后差异表达的基因有98个,其中,已知基因88个,未知基因10个,已知基因中有12个基因上调,76个基因下调.结论:螺旋藻复方有效部位配方对慢性运动性疲劳大鼠脑组织基因表达具有多方面的调控作用,从分子水平阐释了螺旋藻复方有效部位配方抗中枢疲劳的药理作用机制.
目的:研究螺鏇藻複方有效部位配方對慢性運動性疲勞大鼠腦組織基因錶達譜的影響,探索螺鏇藻複方有效部位配方抗疲勞的分子藥理作用機製.方法:分彆從慢性運動性疲勞模型組、螺鏇藻複方有效部位配方治療組SD大鼠的腦組織中抽提總RNA,Cy3、Cy5熒光標記,反轉錄分彆閤成cDNA探針後,與含有4096箇大鼠基因的BiostarR-20S基因錶達譜芯片雜交,Genepix 4000B掃描儀掃描,GenePixPro 3.0軟件分析錶達信號.結果:螺鏇藻複方有效部位配方治療後差異錶達的基因有98箇,其中,已知基因88箇,未知基因10箇,已知基因中有12箇基因上調,76箇基因下調.結論:螺鏇藻複方有效部位配方對慢性運動性疲勞大鼠腦組織基因錶達具有多方麵的調控作用,從分子水平闡釋瞭螺鏇藻複方有效部位配方抗中樞疲勞的藥理作用機製.
목적:연구라선조복방유효부위배방대만성운동성피로대서뇌조직기인표체보적영향,탐색라선조복방유효부위배방항피로적분자약리작용궤제.방법:분별종만성운동성피로모형조、라선조복방유효부위배방치료조SD대서적뇌조직중추제총RNA,Cy3、Cy5형광표기,반전록분별합성cDNA탐침후,여함유4096개대서기인적BiostarR-20S기인표체보심편잡교,Genepix 4000B소묘의소묘,GenePixPro 3.0연건분석표체신호.결과:라선조복방유효부위배방치료후차이표체적기인유98개,기중,이지기인88개,미지기인10개,이지기인중유12개기인상조,76개기인하조.결론:라선조복방유효부위배방대만성운동성피로대서뇌조직기인표체구유다방면적조공작용,종분자수평천석료라선조복방유효부위배방항중추피로적약리작용궤제.
This paper studies the pharmacologic mechanism of SACP＇ACC in antagonizing chronic exercise fatigue,explores its＇ effect on gene expression profile in brain of rats with chronic exercise fatigue. Total RNAs are isolated from rats with chronic exercise fatigue. The mRNAs are reversal transcribed to cDNA with incorporation of fluorescent Cy3 dUTP to prepare hybridization probes. The PCR products of 4096 genes are spotted on the chip after a serial treatment. The mixed probes are hybridized to the cDNA microarray. Genepix 4000B and Gene Pix Pro 3. 0 software are used to scan and analyze the fluorescent signals. The result shows that in the group treated with SACP＇ACC, 98 genes have expression profiles difference to that of in the model group in brain tissue ,in which 88 genes are known, 10 genes are unknown. In known genes, 12 are regulated up and 76 down. It concludes that SACP＇ACC has regulatory effect on gene exression in rats with chronic exercise fatigue, which elucidates part of pharmacologic mechanism of molecular level.
