韶关学院学报
韶關學院學報
소관학원학보
Journal of Shaoguan University(Social Science Edition)
2005年
12期
74~77
,共null页
包英华 白音 王羽梅 粟艳梅 罗靖冬 余红媛
包英華 白音 王羽梅 粟豔梅 囉靖鼕 餘紅媛
포영화 백음 왕우매 속염매 라정동 여홍원
双青35号菜豆 不同外植体 再生体系
雙青35號菜豆 不同外植體 再生體繫
쌍청35호채두 불동외식체 재생체계
Phaseolus vulgaris cv. “Shuangqing 35” ;different explants; regeneration system
报道了双青35号菜豆不同外植体在含有不同激素的培养基上诱导、分化及生根情况,并建立其高效再生体系.研究结果表明:愈伤组织诱导和增殖培养基为MS+6-BA2.0 mg/L+NAA2.0 mg/L,不定芽再生培养基为MS+6-BA2.0mg/L+NAA0.2 mg/L,生根培养基为1/2MS+IAB3.0 mg/L+0.1%活性炭是较佳的.
報道瞭雙青35號菜豆不同外植體在含有不同激素的培養基上誘導、分化及生根情況,併建立其高效再生體繫.研究結果錶明:愈傷組織誘導和增殖培養基為MS+6-BA2.0 mg/L+NAA2.0 mg/L,不定芽再生培養基為MS+6-BA2.0mg/L+NAA0.2 mg/L,生根培養基為1/2MS+IAB3.0 mg/L+0.1%活性炭是較佳的.
보도료쌍청35호채두불동외식체재함유불동격소적배양기상유도、분화급생근정황,병건립기고효재생체계.연구결과표명:유상조직유도화증식배양기위MS+6-BA2.0 mg/L+NAA2.0 mg/L,불정아재생배양기위MS+6-BA2.0mg/L+NAA0.2 mg/L,생근배양기위1/2MS+IAB3.0 mg/L+0.1%활성탄시교가적.
Differentiation and rooting of different explants of Phaseolus vulgaris cv. “Shuangqing 35” were described on different medium in which contained different hormones and a high efficient regeneration system was established. The result showed that the most suitable medium of inducing and proliferating callus is MS ＋ 6 - BA 2.0 mg/L ＋ NAA 2.0 mg/L, medium of regenerating adventitious bud is MS ＋ 6 - BA 2.0 mg/L ＋ NAA0.2 mg/L, rooting medium is 1/2MS ＋ IAB3. 0 mg/L ＋ 0.1% mg/L active carbon.
