CAJ | 학술논문

2月龄雌性SD大鼠进行十周大强度跑台运动后出现运动性动情周期抑制现象，以模拟长期耐力运动训练后女运动员出现的运动性闭经现象，酶法测定下丘脑谷氨酸的含量，RT—PCR法测定大鼠下丘脑GnRH mRNA表达。结果显示，与对照组相比，十周大强度跑台运动致运动性动情周期抑制大鼠血清雌、孕激素水平降低，下丘脑谷氨酸含量降低，下丘脑GnRH mRNA的表达降低。提示十周大强度跑台运动引起下丘脑GnRH的转录功能降低是运动性动情周期抑制产生的重要原因，下丘脑谷氨酸含量的降低可能是影响下丘脑GnRH转录功能的重要因素。
2월령자성SD대서진행십주대강도포태운동후출현운동성동정주기억제현상，이모의장기내력운동훈련후녀운동원출현적운동성폐경현상，매법측정하구뇌곡안산적함량，RT—PCR법측정대서하구뇌GnRH mRNA표체。결과현시，여대조조상비，십주대강도포태운동치운동성동정주기억제대서혈청자、잉격소수평강저，하구뇌곡안산함량강저，하구뇌GnRH mRNA적표체강저。제시십주대강도포태운동인기하구뇌GnRH적전록공능강저시운동성동정주기억제산생적중요원인，하구뇌곡안산함량적강저가능시영향하구뇌GnRH전록공능적중요인소。
2 - month female SD rats were carried with high intensity training in treadmill for 10 weeks. After training, rats displayed that their estrous cycle were inhibited to simulate phenomena of athlete secondary amenorrhea. The content of glutamate in hypothalamus was analyzed by enzyme. The level of GnRH mRNA in hypothalamus was analyzed by RT - PCR. The results showed that high intensity exercise induced most of rots＇ estrons cycle inhibited. The level of estrogen and progesterone in high intensity group were decreased than that in control. The content of glutamate in hypothalamus was lower in high intensity group than that in control. And expression of GnRH mRNA in hypothalamus of high intensity group was reduced than that in control. Those results indicated that long- time high intensity exercise would affect transcription of GnRH in hypothalamus, which is the important cause that inhibited estrous cycle. And the decreasing content of glutamate in typothalamus was one of the important reasons on transcription of GnRH in hypothalamus.