CAJ | 학술논문

通过在不同运动负荷的运动训练状态下，观察大鼠肾脏组织COI Ⅳ、Laminin蛋白、MMP-9酶的表达和尿TP排泄率的变化，以及不同的运动负荷对MMP-9、TIMP-1和CoI Ⅳ mRNA基因表达的影响，研究不同负荷的运动训练对大鼠肾脏ECM、MMP-9／TIMP-1的影响以及MMP-9／TIMP-1的变化在导致肾脏组织结构、生理功能损害上的作用，进一步探讨运动性蛋白尿的产生机制。实验结果表明：经过8周的过度训练后，c组大鼠肾脏COI Ⅳ、Laminin相对于A、B两组出现重度阳性表达，具有显著性差异：CoI Ⅳ、MMP-9、TIMP-1mRNA表达显著增强，TIMP-1mRNA的表达上调了225％显著大于MMP-9mRNA表达上调147％。且尿TP排泄率也显著升高。说明过度运动训练导致ECM产生增加，降解能力下降，致使肾小球及肾小管基底膜ECM异常代谢、沉积和肾间质纤维化的发生和加剧，肾脏组织超微结构及生理功能受到损害。
통과재불동운동부하적운동훈련상태하，관찰대서신장조직COI Ⅳ、Laminin단백、MMP-9매적표체화뇨TP배설솔적변화，이급불동적운동부하대MMP-9、TIMP-1화CoI Ⅳ mRNA기인표체적영향，연구불동부하적운동훈련대대서신장ECM、MMP-9／TIMP-1적영향이급MMP-9／TIMP-1적변화재도치신장조직결구、생리공능손해상적작용，진일보탐토운동성단백뇨적산생궤제。실험결과표명：경과8주적과도훈련후，c조대서신장COI Ⅳ、Laminin상대우A、B량조출현중도양성표체，구유현저성차이：CoI Ⅳ、MMP-9、TIMP-1mRNA표체현저증강，TIMP-1mRNA적표체상조료225％현저대우MMP-9mRNA표체상조147％。차뇨TP배설솔야현저승고。설명과도운동훈련도치ECM산생증가，강해능력하강，치사신소구급신소관기저막ECM이상대사、침적화신간질섬유화적발생화가극，신장조직초미결구급생리공능수도손해。
In order to explore influences of different exercise intensity on renal ECM, MMP-9/TIMP-1 expressin, effects of changes of MMP-9/TIMP-1 on the harmful function to renal tissue structure and physiological function, therefore having a further study in the mechanism of the cause of exercise-induced urinary protein, the Col Ⅳ, LA,MMP-9 expression , the changes of urinary TP excretion rate, and MMP-9 , TIMP-1 and Col Ⅳm RNA expression were tested at different exercise intensity. The results of this study indicated that after 8 weeks over-load training, Col Ⅳ, MMP-9, LA expression, Col Ⅳ,MMP-9, TIMP-1m RNA expression and urinary TP excretion rate increased significantly in group C than in group A and B. TIMP-1m RNA expression in group C increases by 225%. It showed that over-load training induced the increase of ECM, decreased the degradation ability, and as a result it caused the abnormal metabolism, accumulation of ECM and damage to renal micro-structure and physiological function.