中国体育科技
中國體育科技
중국체육과기
China Sport Science and Technology
2007年
2期
83~86
,共null页
安玉香 夏书红 刘金海 王岸新
安玉香 夏書紅 劉金海 王岸新
안옥향 하서홍 류금해 왕안신
运动性蛋白尿 过度训练 肾脏 凋亡 粉防己碱 鼠 动物实验
運動性蛋白尿 過度訓練 腎髒 凋亡 粉防己堿 鼠 動物實驗
운동성단백뇨 과도훈련 신장 조망 분방기감 서 동물실험
exercise albumin ria ; overtra ining ; kidney; tetra ndrine ; rat ; animal experiment
目的：研究粉防己碱对运动性蛋白尿发生时肾脏细胞凋亡的干预作用。方法：将大量购进的符合实验要求的雄性Wistar大鼠先按每组15只随机分为安静对照组和安静干预组，其余大鼠每天行负重游泳训练连续2h，待复制出确切的运动性蛋白尿模型后随机纳入实验，并按每组15只将其分为过度训练对照纽和过度训练干预组。安静对照组和过度训练对照组每日用pH7．4的磷酸盐缓冲液1．5ml／kg灌胃，安静干预组和过度训练干预组每日灌注粉防己碱30mg／kg，连续用药10d。安静对照组和安静干预组给药期间安静自由喂养，而过度训练对照组和过度训练干预组则需在给药后4h按原方案行负重游泳训练2h。用末端标记法检测各组大鼠肾组织细胞凋亡，采用图像分析仪计算肾脏细胞凋亡率。结果：过度训练对照组尿蛋白持续增高，肾皮质可见大量凋亡细胞，凋亡百分率明显高于其他各组（P〈0．01）。过度训练干预组未见尿蛋白持续增高现象，肾组织仅见少量凋亡细胞数，凋亡百分率虽高于安静干预组与安静对照组，但仍明显低于过度训练对照组（P〈0．01）。结论：过度训练可导致尿蛋白持续增加和肾脏细胞凋亡，粉防己碱可阻止过量运动所致的肾脏细胞凋亡，进而减轻运动性蛋白尿，对运动性肾损伤有保护作用。
目的：研究粉防己堿對運動性蛋白尿髮生時腎髒細胞凋亡的榦預作用。方法：將大量購進的符閤實驗要求的雄性Wistar大鼠先按每組15隻隨機分為安靜對照組和安靜榦預組，其餘大鼠每天行負重遊泳訓練連續2h，待複製齣確切的運動性蛋白尿模型後隨機納入實驗，併按每組15隻將其分為過度訓練對照紐和過度訓練榦預組。安靜對照組和過度訓練對照組每日用pH7．4的燐痠鹽緩遲液1．5ml／kg灌胃，安靜榦預組和過度訓練榦預組每日灌註粉防己堿30mg／kg，連續用藥10d。安靜對照組和安靜榦預組給藥期間安靜自由餵養，而過度訓練對照組和過度訓練榦預組則需在給藥後4h按原方案行負重遊泳訓練2h。用末耑標記法檢測各組大鼠腎組織細胞凋亡，採用圖像分析儀計算腎髒細胞凋亡率。結果：過度訓練對照組尿蛋白持續增高，腎皮質可見大量凋亡細胞，凋亡百分率明顯高于其他各組（P〈0．01）。過度訓練榦預組未見尿蛋白持續增高現象，腎組織僅見少量凋亡細胞數，凋亡百分率雖高于安靜榦預組與安靜對照組，但仍明顯低于過度訓練對照組（P〈0．01）。結論：過度訓練可導緻尿蛋白持續增加和腎髒細胞凋亡，粉防己堿可阻止過量運動所緻的腎髒細胞凋亡，進而減輕運動性蛋白尿，對運動性腎損傷有保護作用。
목적：연구분방기감대운동성단백뇨발생시신장세포조망적간예작용。방법：장대량구진적부합실험요구적웅성Wistar대서선안매조15지수궤분위안정대조조화안정간예조，기여대서매천행부중유영훈련련속2h，대복제출학절적운동성단백뇨모형후수궤납입실험，병안매조15지장기분위과도훈련대조뉴화과도훈련간예조。안정대조조화과도훈련대조조매일용pH7．4적린산염완충액1．5ml／kg관위，안정간예조화과도훈련간예조매일관주분방기감30mg／kg，련속용약10d。안정대조조화안정간예조급약기간안정자유위양，이과도훈련대조조화과도훈련간예조칙수재급약후4h안원방안행부중유영훈련2h。용말단표기법검측각조대서신조직세포조망，채용도상분석의계산신장세포조망솔。결과：과도훈련대조조뇨단백지속증고，신피질가견대량조망세포，조망백분솔명현고우기타각조（P〈0．01）。과도훈련간예조미견뇨단백지속증고현상，신조직부견소량조망세포수，조망백분솔수고우안정간예조여안정대조조，단잉명현저우과도훈련대조조（P〈0．01）。결론：과도훈련가도치뇨단백지속증가화신장세포조망，분방기감가조지과량운동소치적신장세포조망，진이감경운동성단백뇨，대운동성신손상유보호작용。
The purpose of this study was to analyze the intervention of tetrandrine on the apoptosis of renal cell with exercise albuminria. Mass purchasing male Wistar rats according with the requirement of this experiment was randomly divided into sedentary control group and sedentary interventional group with 15 rats per group, the remainder did loaded-swimming every day for 2 hours, until replicating the model of exercise albuminria. The rats with exercise albuminria were randomly divided into overtraining control group and overtraining interventional group with 15 rats per group. Sedentary control group and overtraining control group with pH7.4 PBS 1.5ml/ kg per day, perfusion, sedentary interventional group and overtraining interventional group with tetrandrine 30mg/kg per day, perfusion, with the group and sedentary interventional group peaceful medicine for 10 days. The sedentary control feeding, while the overtraining control group and overtraining interventional group doing loaded-swimming every day fore 2 hours after with the medicine 4 hours. Detecting the in situ cell apoptosis by TUNEL and calculating the ratio of renal cell apoptosis by image dissector. The result shows that in overtraining control group,the urinary protein increased significantly in concomitant,the mass cell of apoptosis have already been clearly observed on the Proximal tubule, the ratio of renal cell apoptosis increased significantly that in other group（P〈0.01）. The overtraining interventional group without the phenomena of urinary protein increased, the ratio of renal cell apoptosis higher than sedentary control group and sedentary interventional group,those effects still remains have very significantly difference compared with the overtraining control group（P〈0. 01）. Conclusions It concludes that overtraining can lead to increase the urinary protein in concomitant and evoke apoptosis of renal cell. Tetrandrine is able to protect the renal tubular epithelial cell apoptosis induced hy overtraining. The above results can indicate that tetrandrine has antagonism when renal injury induced by violent movement.
