广东教育学院学报
廣東教育學院學報
엄동교육학원학보
Journal of Guangdong Education Institute
2007年
3期
64~68
,共null页
针禾属植物 羽毛针禾 SDS法DNA提取 CTAB法DNA提取 随机扩增多态DNA 微卫星
針禾屬植物 羽毛針禾 SDS法DNA提取 CTAB法DNA提取 隨機擴增多態DNA 微衛星
침화속식물 우모침화 SDS법DNA제취 CTAB법DNA제취 수궤확증다태DNA 미위성
Stipagrosits De Winter; St;pagrosits pennata; SDS DNA extraction; CTAB DNA extraction; Random Amplified Polymorphic DNA;SSR
传统的CTABDNA提取法步骤多．较烦琐，DNA产率低，而且由于酚、单宁、色素、多糖很难完全去除，容易影响随机扩增多态、微卫星等标记工作的效率．采用SDS裂解法提取了针禾属植物幼嫩叶片的基因组DNA，所获得的DNA可用于PCR反应．并且在针禾属植物的研究中得到了较好的结果．
傳統的CTABDNA提取法步驟多．較煩瑣，DNA產率低，而且由于酚、單寧、色素、多糖很難完全去除，容易影響隨機擴增多態、微衛星等標記工作的效率．採用SDS裂解法提取瞭針禾屬植物幼嫩葉片的基因組DNA，所穫得的DNA可用于PCR反應．併且在針禾屬植物的研究中得到瞭較好的結果．
전통적CTABDNA제취법보취다．교번쇄，DNA산솔저，이차유우분、단저、색소、다당흔난완전거제，용역영향수궤확증다태、미위성등표기공작적효솔．채용SDS렬해법제취료침화속식물유눈협편적기인조DNA，소획득적DNA가용우PCR반응．병차재침화속식물적연구중득도료교호적결과．
The traditional CTAB DNA extract method is troublesome with many steps and the gross Genomic DNA extracted by it is very low. High content of phenolic terpenoids, tannin, pigments, polysaccharide, etc. usually affect the activity of Taq and lead to the failure of PCR reaction. Genomic DNA of tender leaf is extracted in SDS method. The genomic DNA can be applied as template DNA in PCR reaction. The results suggested that SDS procedure was suitable for Stipagrosits genome DNA extraction.
