泉州师范学院学报
泉州師範學院學報
천주사범학원학보
Journal of Quanzhou Normal College
2006年
6期
90~94
,共null页
陈巧 黄周英 鄢绯寰 吕良炬
陳巧 黃週英 鄢緋寰 呂良炬
진교 황주영 언비환 려량거
SSR福建家鸭 遗传多样性
SSR福建傢鴨 遺傳多樣性
SSR복건가압 유전다양성
SSR;domestic duck in Fujian province; genetic diversity
利用SSR技术分析福建省家鸭地方良种金定鸭、山麻鸭、连城白鸭和野鸭绿头鸭、斑嘴鸭基因组DNA的多态性,在21个位点共扩增得到421条带,其中多态性条带213条,约占总数的50.6%.多态性位点比率在33.3%~84.6%之间.运用简单匹配系数法计算了品种(系)间的相似性系数和遗传距离,家鸭品种间的遗传距离在0.043~0.251范围内,绿头鸭、斑嘴鸭与家鸭品种(系)间的遗传距离分别在0.203(金定鸭Ⅰ系)~0.232(金定鸭Ⅱ系)和0.236(山麻鸭)~0.317(金定鸭Ⅱ系)范围内.连城白鸭与金定鸭3个品系间的遗传距离介于0.232至0.251之间,山麻鸭与金定鸭3品系间的遗传距离在0.239至0.248范围内.而连城白鸭与山麻鸭间的遗传距离为0.136.
利用SSR技術分析福建省傢鴨地方良種金定鴨、山痳鴨、連城白鴨和野鴨綠頭鴨、斑嘴鴨基因組DNA的多態性,在21箇位點共擴增得到421條帶,其中多態性條帶213條,約佔總數的50.6%.多態性位點比率在33.3%~84.6%之間.運用簡單匹配繫數法計算瞭品種(繫)間的相似性繫數和遺傳距離,傢鴨品種間的遺傳距離在0.043~0.251範圍內,綠頭鴨、斑嘴鴨與傢鴨品種(繫)間的遺傳距離分彆在0.203(金定鴨Ⅰ繫)~0.232(金定鴨Ⅱ繫)和0.236(山痳鴨)~0.317(金定鴨Ⅱ繫)範圍內.連城白鴨與金定鴨3箇品繫間的遺傳距離介于0.232至0.251之間,山痳鴨與金定鴨3品繫間的遺傳距離在0.239至0.248範圍內.而連城白鴨與山痳鴨間的遺傳距離為0.136.
이용SSR기술분석복건성가압지방량충금정압、산마압、련성백압화야압록두압、반취압기인조DNA적다태성,재21개위점공확증득도421조대,기중다태성조대213조,약점총수적50.6%.다태성위점비솔재33.3%~84.6%지간.운용간단필배계수법계산료품충(계)간적상사성계수화유전거리,가압품충간적유전거리재0.043~0.251범위내,록두압、반취압여가압품충(계)간적유전거리분별재0.203(금정압Ⅰ계)~0.232(금정압Ⅱ계)화0.236(산마압)~0.317(금정압Ⅱ계)범위내.련성백압여금정압3개품계간적유전거리개우0.232지0.251지간,산마압여금정압3품계간적유전거리재0.239지0.248범위내.이련성백압여산마압간적유전거리위0.136.
Simple sequence repeat technique was used to estimate the genomic DNA polymorphism among three famous endemic breeds of domestic ducks (Jinding duck, Lianchen white duck,Longyan Shanma duck)and two species of wild duck( Anas Platyrhyncho,and Anas Poecilorhyncha ) in Fujian province. 21 SSR primer pairs detected a total of 421 fragments. Two hundred and thirteen bands, representing 50.6 % of total fragments, were polymorphic. The proportion of polymorphic loci ranged from 33. 3%%to 84. 6%. Simple matching coefficients (SM)was calculated to manifest the genetic similarity and distance. The genetic distance between domestic ducks was 0. 043~0. 251 ,while between domestic ducks and the wild ducks was 0. 203~ 0. 232( Arms Platyrhynchos ) and 0. 236~0. 317 (Arms Poecilorhyncha ) respectively. The distances range from 0. 232 to 0.251 between Lianchen white duck and three strains of Jinding duck,while range from 0. 239 to 0. 248 between Shanma duck and the 3 ones. The genetic distance between Lianchen duck and Shanma duck is 0. 136.