岳阳职业技术学院学报
嶽暘職業技術學院學報
악양직업기술학원학보
Yueyang Vocational Technical College
2006年
5期
69~70
,共null页
秀珍菇 原生质体 分离 再生
秀珍菇 原生質體 分離 再生
수진고 원생질체 분리 재생
pleurotus cornucopiae （Paul.：Pers.） rolland; protoplast; isolation; regeneration
通过对秀珍菇原生质体的分离与再生的影响因素的试验研究,表明以0.6M甘露醇为渗稳剂用1%纤维素酶＋1%蜗牛酶,30℃酶解3～4小时原生质体产生最高,原生质体再生则以0.6M甘露醇或0.6M蔗糖为渗稳剂较好。
通過對秀珍菇原生質體的分離與再生的影響因素的試驗研究,錶明以0.6M甘露醇為滲穩劑用1%纖維素酶＋1%蝸牛酶,30℃酶解3～4小時原生質體產生最高,原生質體再生則以0.6M甘露醇或0.6M蔗糖為滲穩劑較好。
통과대수진고원생질체적분리여재생적영향인소적시험연구,표명이0.6M감로순위삼은제용1%섬유소매＋1%와우매,30℃매해3～4소시원생질체산생최고,원생질체재생칙이0.6M감로순혹0.6M자당위삼은제교호。
Through the experiments on the facts which affect the isolation and regeneration of Pleurotus comucopiae （Paul.：Pers.）Rolland protoplast, the result shows that the rate of protoplast was produced to the top by means of osmotic stabilizer of 0.6 M annitol, 1% cellulose and 1% novozyme, after 3-4 hours with 30℃. And it turns out that 0.6 annitol or 0.6 sucrose is used as osmotic stabilizer to make protoplast regeneration.
