衡水学院学报
衡水學院學報
형수학원학보
Journal of Hengshui University
2007年
1期
27~29
,共null页
蝴蝶兰 快速繁殖 原球茎
蝴蝶蘭 快速繁殖 原毬莖
호접란 쾌속번식 원구경
Phalaenopsis ; rapid propagation ; protocorm - like body
以蝴蝶兰叶片为外植体进行了快繁研究.结果表明:活性炭配合转瓶能有效地减轻叶片的褐变现象.在MS培养基中添加10%的香蕉汁可以明显提高诱导率.植物激素显著影响原球茎的形成,在MS+6-BA2mg/L+NAA0.2mg/L的培养基中,原球茎的诱导率最高.原球茎在诱导培养基上增殖30代时,在无激素的培养基中进行分化,获得的幼苗生长健壮,变异率极低.
以蝴蝶蘭葉片為外植體進行瞭快繁研究.結果錶明:活性炭配閤轉瓶能有效地減輕葉片的褐變現象.在MS培養基中添加10%的香蕉汁可以明顯提高誘導率.植物激素顯著影響原毬莖的形成,在MS+6-BA2mg/L+NAA0.2mg/L的培養基中,原毬莖的誘導率最高.原毬莖在誘導培養基上增殖30代時,在無激素的培養基中進行分化,穫得的幼苗生長健壯,變異率極低.
이호접란협편위외식체진행료쾌번연구.결과표명:활성탄배합전병능유효지감경협편적갈변현상.재MS배양기중첨가10%적향초즙가이명현제고유도솔.식물격소현저영향원구경적형성,재MS+6-BA2mg/L+NAA0.2mg/L적배양기중,원구경적유도솔최고.원구경재유도배양기상증식30대시,재무격소적배양기중진행분화,획득적유묘생장건장,변이솔겁저.
Rapid propagation on leaf explants of Phalanopsis was studied. The results showed that ; active cardon and shifting leaf explants to another new medium were effective for reducing the explants from being brown. When 10% banana juice was added into Mstnedium, the inducing rate of the protocorm - like body was obviously accelerated. 6 - BA and NAA could stimulate protocorm - like body induction, and the effective results could be obtained on MS + 6 - BA 2mg/L + NAA0.2mg/L. When protocorm - like bodies were proliferated for 30 times on inducing mediums, it was the time to transform the protocorm - like bodies on MS mediums. The transplants were growing well; the rate of varying was extremely low.