天津商学院学报
天津商學院學報
천진상학원학보
Journal of Tianjin University of Commerce
2007年
6期
17~20
,共null页
分光光度法 联苯胺 盐酸萘乙二胺 亚硝酸根 测定
分光光度法 聯苯胺 鹽痠萘乙二胺 亞硝痠根 測定
분광광도법 련분알 염산내을이알 아초산근 측정
speetrophotometry; benzidine ; hydrochloric acid naphthyl ethylenediamine; nitrite; determination
报道了一种测定食品中亚硝酸盐的新方法。在酸性条件下，亚硝酸盐与联苯胺重氮化，再与盐酸萘乙二胺偶联生成蓝色产物，该产物在578nm处有最大吸收值，并由此建立了一种简单、快速、灵敏的亚硝酸盐测定方法。在优化实验条件下，亚硝酸根的浓度在0～0．8μg／mL范围内服从比尔定律，方法的检出限和表观摩尔吸光系数分别为7ng／mL和4．99×10^4L／（mol·cm）。本法用于测定各种食品如香肠、火腿、午餐肉、榨菜中痕量亚硝酸根的含量，结果与国标法一致。并对反应机理进行了讨论。
報道瞭一種測定食品中亞硝痠鹽的新方法。在痠性條件下，亞硝痠鹽與聯苯胺重氮化，再與鹽痠萘乙二胺偶聯生成藍色產物，該產物在578nm處有最大吸收值，併由此建立瞭一種簡單、快速、靈敏的亞硝痠鹽測定方法。在優化實驗條件下，亞硝痠根的濃度在0～0．8μg／mL範圍內服從比爾定律，方法的檢齣限和錶觀摩爾吸光繫數分彆為7ng／mL和4．99×10^4L／（mol·cm）。本法用于測定各種食品如香腸、火腿、午餐肉、榨菜中痕量亞硝痠根的含量，結果與國標法一緻。併對反應機理進行瞭討論。
보도료일충측정식품중아초산염적신방법。재산성조건하，아초산염여련분알중담화，재여염산내을이알우련생성람색산물，해산물재578nm처유최대흡수치，병유차건립료일충간단、쾌속、령민적아초산염측정방법。재우화실험조건하，아초산근적농도재0～0．8μg／mL범위내복종비이정률，방법적검출한화표관마이흡광계수분별위7ng／mL화4．99×10^4L／（mol·cm）。본법용우측정각충식품여향장、화퇴、오찬육、자채중흔량아초산근적함량，결과여국표법일치。병대반응궤리진행료토론。
A rapid, simple, sensitive spectrophotometric determination of nitrite using new diazotizing and coupling reagents is described. The method is based on a diazotion-eoupling reaction between benzidine and hydrochloric acid naphthyl ethylenediamine in a hydrochloric acid medium. This increase in absorbanee is directly proportional to nitrite concentration obeying Beer＇ s law. Linear calibration graphs are obtained for 0 -0.8 μg/ml nitrite at 578nm. The molar absorptivity and the detection limit are found to be 4.99 × 10^4L（ mol · com） and 7ng/mL. The method is applied to determine nitrite in sausage, ham, cured ham and pickle samples with satisfactory results. Finally, the mechanism of the reaction is discussed.
