CAJ | 학술논문

目的：探讨有氧耐力训练对不同周龄大鼠骨骼肌代谢酶活性的影响，从而为确定提高有氧运动能力的最适耐力训练时间提供理论依据。方法：健康雄性wistar大鼠48只，根据开始游泳训练的周龄不同分为4周龄组、8周龄组和12周龄组，每组16只（每组分别随机设安静对照组8只），建立有氧耐力运动训练模型。对照组常规饲养，不运动。训练组均进行中等强度游泳训练。首先进行4天适应性训练，每天25min，正式训练第1周50 min／天，以后每周增加10 min／天，直至120min。每周训练6天，休息1天，造模总时闻为8周。用酶组织化学法分剐测定犬鼠股四头肌琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）、三磷酸腺苷酶（ATPase）的活性争糖原（PAS）的含量。结果：8周训练后，与对照组相比，4周龄组、8周龄组大鼠骨骼肌SDH活性增高且差异非常显著（P〈0．01）；12周龄组与对照组相比SDH活性增高且差异显著（P〈0．05）。与对照组相比，4周龄组、8周龄组大鼠骨骼肌LDH和ATPase活性降低且差异显著，而12周龄组无明显变化。与对照组相比，4周龄组、8周龄组、12周龄组大鼠骨骼肌PAS活性增高且差异显著（P〈0．05）。结论：有氧耐力训练一方面可提高4周龄组、8周龄组、12周龄组大鼠骨骼肌有氧代谢能力，且4周龄组、8周龄组效果更突出。另一方面它降低了4周龄组、8周龄组大鼠骨骼肌糖酵解供能能力，而对12周龄组大鼠骨骼肌糖酵解供能能力的影响尚不确定。提示早期（幼儿时期）进行有氧耐力训练可能是提高有氧运动能力的最佳时期。
목적：탐토유양내력훈련대불동주령대서골격기대사매활성적영향，종이위학정제고유양운동능력적최괄내력훈련시간제공이론의거。방법：건강웅성wistar대서48지，근거개시유영훈련적주령불동분위4주령조、8주령조화12주령조，매조16지（매조분별수궤설안정대조조8지），건립유양내력운동훈련모형。대조조상규사양，불운동。훈련조균진행중등강도유영훈련。수선진행4천괄응성훈련，매천25min，정식훈련제1주50 min／천，이후매주증가10 min／천，직지120min。매주훈련6천，휴식1천，조모총시문위8주。용매조직화학법분과측정견서고사두기호박산탈경매（SDH）、유산탈경매（LDH）、삼린산선감매（ATPase）적활성쟁당원（PAS）적함량。결과：8주훈련후，여대조조상비，4주령조、8주령조대서골격기SDH활성증고차차이비상현저（P〈0．01）；12주령조여대조조상비SDH활성증고차차이현저（P〈0．05）。여대조조상비，4주령조、8주령조대서골격기LDH화ATPase활성강저차차이현저，이12주령조무명현변화。여대조조상비，4주령조、8주령조、12주령조대서골격기PAS활성증고차차이현저（P〈0．05）。결론：유양내력훈련일방면가제고4주령조、8주령조、12주령조대서골격기유양대사능력，차4주령조、8주령조효과경돌출。령일방면타강저료4주령조、8주령조대서골격기당효해공능능력，이대12주령조대서골격기당효해공능능력적영향상불학정。제시조기（유인시기）진행유양내력훈련가능시제고유양운동능력적최가시기。
Objective：To provide theoretical evidence for ensuring the most suitable time of endurance waning for developing the aerobic capacity by studying the effect on the active of metabolic enzyme of skeletal muscle of mice at different weeks old. Methods ： Total 48 male mice were at three different levels of weeks old, 16 mice were 4 weeks old, as many as 8 weeks old and 12 weeks old. Each the 16 mice were randomly divided into tow groups, One group swam at middling strength, and the other group was as a control group. Each swimming group swam for 25 minutes a day in the first four days as fitness. And then the swimming groups swam for 50 minutes a day. Every week, the time of swimming was lengthened 10 minutes,and after every six days, the mice swam had a rest for a day. The total time for training was 8 weeks. Enzymohistochemistry was used to study the activity of SDH,LDH, ATPase and the amount of PAS in skeletal muscle of mice. Results ： After 8 weeks training, compared with the control groups, the activity of SDH in both 4 weeks old swimming group and 8 weeks old swimming group were highly significantly increased（P 〈0.01）,the activity of SDH in 12 weeks old swimming group was significantly increased （P 〈 0.05） ; Compared with the control groups, the activity of LDH and ATPase in both 4 weeks old swimming group and 8 weeks old swimming group were significantly decreased （P 〈 0.05）,but no difference was existed between the tow groups at 12 weeks old;Compared with the control groups, the amount of PAS in all swimming groups were significantly increased （ P 〈 0.05）. Conclusion ： The endurance training helped to develop the aerobic capacity of mice in all three swimming groups, especially both the 4 weeks old swimming group and 8 weeks old swimming group. On the other hand, the endurance training reduced the glycolysis capacity of skeletal muscle of mice in both the 4 weeks old swimming group and 8 weeks old swimming group, but the effect of the endurance training on glycolysis capacity of skeletal muscle of mice in the 12 weeks old group was not sure. Therefore, young children period was the best time to have aerobic endurance training for developing the aerobic capacity.