CAJ | 학술논문

目的：探讨过度训练对肠道细胞免疫功能的影响以及谷氨酰胺的干预作用和机制。方法：实验大鼠随机分为对照组、过度训练组、谷氨酰胺干预组。过度训练组大鼠进行每周6次、共9周的力竭性运动训练。谷氨酰胺干预组大鼠,在大负荷运动之前按1.5g/kgBW剂量补充谷氨酰胺。9周后,检测各组大鼠小肠组织sIgA的含量及小肠CD4＋、CD8＋T细胞数量和Gln以及血浆皮质酮含量。结果：与对照组比较,过度训练组大鼠小肠组织中CD4＋T细胞数量、CD4＋/CD8＋比值显著下降,小肠CD8＋T细胞数量显著升高;且小肠中Gln含量显著下降,血浆皮质酮含量显著升高。与过度训练组相比,Gln干预组小肠CD4＋T细胞数量、CD4^＋/CD8^＋的比值显著升高,CD8^＋T细胞数量显著降低,血浆皮质酮含量显著降低。结论：过度训练导致大鼠肠道细胞免疫功能显著下降;而过度训练大鼠小肠Gln含量下降和皮质酮的分泌增多,可能是过度训练引起肠道免疫功能降低的主要机制。补充Gln可改善长期大负荷运动训练大鼠肠道细胞免疫功能;而Gln的补充有效维持大负荷训练大鼠小肠内Gln含量,降低皮质酮分泌,这可能也是补充Gln防止肠道免疫功能下降的主要机制。
목적：탐토과도훈련대장도세포면역공능적영향이급곡안선알적간예작용화궤제。방법：실험대서수궤분위대조조、과도훈련조、곡안선알간예조。과도훈련조대서진행매주6차、공9주적력갈성운동훈련。곡안선알간예조대서,재대부하운동지전안1.5g/kgBW제량보충곡안선알。9주후,검측각조대서소장조직sIgA적함량급소장CD4＋、CD8＋T세포수량화Gln이급혈장피질동함량。결과：여대조조비교,과도훈련조대서소장조직중CD4＋T세포수량、CD4＋/CD8＋비치현저하강,소장CD8＋T세포수량현저승고;차소장중Gln함량현저하강,혈장피질동함량현저승고。여과도훈련조상비,Gln간예조소장CD4＋T세포수량、CD4^＋/CD8^＋적비치현저승고,CD8^＋T세포수량현저강저,혈장피질동함량현저강저。결론：과도훈련도치대서장도세포면역공능현저하강;이과도훈련대서소장Gln함량하강화피질동적분비증다,가능시과도훈련인기장도면역공능강저적주요궤제。보충Gln가개선장기대부하운동훈련대서장도세포면역공능;이Gln적보충유효유지대부하훈련대서소장내Gln함량,강저피질동분비,저가능야시보충Gln방지장도면역공능하강적주요궤제。
Objective： To explore the effect of overtraining on intestinal cellular immunity in rats and the intervention effect of glutamine and its mechanisms. Method： Experimental rats were randomly divided into control group, overtraining group and glutamine intervention group. The rats in overtraining group were carrying on exhaustive training for 9 weeks, 6 times/week. The rats in glutamine intervention group was supplemented Gln with 1. 5g/kgBW dosage before heavy training. 9 weeks later, Gln content and CD4^＋ , CD8^＋ quantity in small intestine and plasma cortisone content were examined. Result：Compared with the control group, in the overtraining group, intestinal CD4^＋ quantity and CD4^＋/CD8^＋ ratio obviously decreased, CD8^＋ quantity obviously increased. And plasma cortisone content obviously increased. Compared with the overtraining group, in the glutamine intervention group, intestinal CD4^＋ quantity, CD4^＋ / CD8^＋ ratio increased significantly, CD8^＋ quantity obviously reduced, and plasma cortisone content obviously reduced. Conclusion ： Overtraining might cause intestinal cellular immunity in rats to reduce obviously. The significant decrease of intestinal Gln concentration and increase of plasma cortisone concentration in rats with overtraining might he main mechanism that overtraining causes intestinal cellular immunity to reduce. Supplement of Gln could improve intesti- nal cellular immunity in rats with long-term heavyload training. Gln supplement might prevent intestinal Gin content to decrease and reduce the secretion of cortisone in rats with heavy load training. It might be the main intervention mechanism which Gln supplement prevented the decrease of intestinal cellular immunity.