体育科学
體育科學
체육과학
China Sport Science
2008年
12期
56~61
,共null页
史绍蓉 袁爱国 王娟 刘建荣 刘田 李颂
史紹蓉 袁愛國 王娟 劉建榮 劉田 李頌
사소용 원애국 왕연 류건영 류전 리송
运动心脏 左室肌 右室肌 中等强度运动 蛋白质组 差异表达
運動心髒 左室肌 右室肌 中等彊度運動 蛋白質組 差異錶達
운동심장 좌실기 우실기 중등강도운동 단백질조 차이표체
athletic heart ; left ventricular muscle ; right ventricular muscle ; medium intensity training ; proteome ; differential expression
目的:探讨4周中等强度运动后大鼠左、右室肌蛋白质组的差异性表达,初步筛选出心脏在运动反应与适应过程中的目标蛋白质;方法:将18只雄性SD大鼠随机分为实验组和平行对照组(n=9)。实验组经4周中等负荷强度(70%VO2 max-80%VO2 max)跑台训练后,与对照组一起,提取左、右室肌组织的全蛋白样品。进行2-DE蛋白样品分离,采用PD-Quest软件进行图像分析和数据采集。选择运动后差异表达量≥10倍的备选目标蛋白点,进行胶内原位酶解与一、二级质谱鉴定;结果:运动后左、右心室差异表达量≥10倍的蛋白质点共24个,取其中9个点,经一、二级质谱鉴定出ATP合酶偶合因子6、葡萄糖调节蛋白78、烯酰辅酶A水合酶、ATP合酶α亚基、丙酮酸脱氢酶E1α1、硒联蛋白1、类似信号增殖关联蛋白1和丝氨酸精氨酸前体-mRNA拼接因子SR-A1以及一个分子量为116kDa的未知蛋白;结论:1)实验大鼠在心脏重塑过程中,左、右室肌蛋白质组发生了显著的变化。2)成功筛选出在运动医学领域中尚未涉足的8种目标蛋白,其表达量发生的适应性变化表明,在中等强度运动下,会引起心肌能量代谢水平的提高、心肌应激反应与适应能力的增强、血压调节能力的改善等一系列有益变化。
目的:探討4週中等彊度運動後大鼠左、右室肌蛋白質組的差異性錶達,初步篩選齣心髒在運動反應與適應過程中的目標蛋白質;方法:將18隻雄性SD大鼠隨機分為實驗組和平行對照組(n=9)。實驗組經4週中等負荷彊度(70%VO2 max-80%VO2 max)跑檯訓練後,與對照組一起,提取左、右室肌組織的全蛋白樣品。進行2-DE蛋白樣品分離,採用PD-Quest軟件進行圖像分析和數據採集。選擇運動後差異錶達量≥10倍的備選目標蛋白點,進行膠內原位酶解與一、二級質譜鑒定;結果:運動後左、右心室差異錶達量≥10倍的蛋白質點共24箇,取其中9箇點,經一、二級質譜鑒定齣ATP閤酶偶閤因子6、葡萄糖調節蛋白78、烯酰輔酶A水閤酶、ATP閤酶α亞基、丙酮痠脫氫酶E1α1、硒聯蛋白1、類似信號增殖關聯蛋白1和絲氨痠精氨痠前體-mRNA拼接因子SR-A1以及一箇分子量為116kDa的未知蛋白;結論:1)實驗大鼠在心髒重塑過程中,左、右室肌蛋白質組髮生瞭顯著的變化。2)成功篩選齣在運動醫學領域中尚未涉足的8種目標蛋白,其錶達量髮生的適應性變化錶明,在中等彊度運動下,會引起心肌能量代謝水平的提高、心肌應激反應與適應能力的增彊、血壓調節能力的改善等一繫列有益變化。
목적:탐토4주중등강도운동후대서좌、우실기단백질조적차이성표체,초보사선출심장재운동반응여괄응과정중적목표단백질;방법:장18지웅성SD대서수궤분위실험조화평행대조조(n=9)。실험조경4주중등부하강도(70%VO2 max-80%VO2 max)포태훈련후,여대조조일기,제취좌、우실기조직적전단백양품。진행2-DE단백양품분리,채용PD-Quest연건진행도상분석화수거채집。선택운동후차이표체량≥10배적비선목표단백점,진행효내원위매해여일、이급질보감정;결과:운동후좌、우심실차이표체량≥10배적단백질점공24개,취기중9개점,경일、이급질보감정출ATP합매우합인자6、포도당조절단백78、희선보매A수합매、ATP합매α아기、병동산탈경매E1α1、서련단백1、유사신호증식관련단백1화사안산정안산전체-mRNA병접인자SR-A1이급일개분자량위116kDa적미지단백;결론:1)실험대서재심장중소과정중,좌、우실기단백질조발생료현저적변화。2)성공사선출재운동의학영역중상미섭족적8충목표단백,기표체량발생적괄응성변화표명,재중등강도운동하,회인기심기능량대사수평적제고、심기응격반응여괄응능력적증강、혈압조절능력적개선등일계렬유익변화。
Objective: This paper probed into the differential expression of the left and the right ventricular muscle proteome after 4 weeks medium intensity exercise, and filtrating out the objective proteins during the process of the heart reaction and adoption to the exercise. Methods: Dividing 18 male SD rats into exercise and parallel control groups at random(n:9), the exercise group had a 4 weeks treadmill training with medium intensity (70%VO2 max-80%VO2 max), together with the control group, preparing for whole proteins samples of the left and right ventricular muscle, and separating the protein samples by utilizing two-dimensional gel electrophoresis, and analyzing image and collecting data by using PD-Quest. The alternatively objective protein spots whose differential expression volume ≥ 10 folds were selected for in-gel enzymologic extraction and MS/MS identification. Result= There were 24 points expressed ≥10 folds. Taking out 9 of them, and identified 8, they were ATP synthase coupling factor 6,78kDa glucose-regulated protein, Enoyl-CoA hydratase, ATP synthase subunit alpha, pyruvate dehydrogenase E1 alpha 1, selenium-binding protein 1, Signal-induced proliferationassociated 1-like protein 1, Serine arginine-rich pre-mRNA splicing factor SR-A1 and an unknown protein whose Mr was 116 kDa. Conclusion.. 1)During the heart remodeling process of the test groups, the proteome of the left and right ventrieular muscle had a remarkable change. 2)We succeeded in filtrating 8 objective proteins which were not mentioned in the sports medicine area at present, their expression volume had an adaptive changes, it indicated that under the medium intensity training, it could bring a series of well changes such as improving the cardiac muscle energy metabolism level, strengthening the ability of cardiac muscle stress reaction and adaption, and meliorating the blood pressure adjustment capacity.