CAJ | 학술논문

观察不同强度有氧耐力训练后的骨骼肌PPARα／δ表达、相关酶活性、肌纤维类型和运动能力的变化，探究PPARα／δ在提高C57BL／6小鼠骨骼肌有氧耐力中的作用以及训练强度的影响。选用6周龄雄性C57BL／6小鼠45只，随机分为3个实验组，分别为76％VO2max 60min训练组（LS）、86％VO2max60min训练组（HS）和安静对照组（RC）。各运动组进行跑台训练，采用RT-PCR、Westernblot、MATPase染色检测骨骼肌中PPARα／δ、CPT-1、BoAcDH和GPDH的表达及肌纤维类型的变化。结果发现：运动各组骨骼肌PPARα／δ转录和翻译水平较对照组均有显著提高，而HS组均显著高于LS组；各运动组CPT-1、BoAc—DH的蛋白袁达和运动能力较对照组均有显著提高，但运动组间没有显著差异，GPDH的表达没有显著改变；运动各组耐力能力较对照组均有显著增强，但运动组间没有显著差异；各组之间的骨骼肌Ⅰ、Ⅱ型肌纤维组成百分比均没有显著差异。结论：PPARα／δ可能作为靶点在骨骼肌对耐力训练的适应过程中发挥着重要作用，高强度训练对于这种适应过程的影响较大；运动并没有导致骨骼肌纤维类型发生显著变化。
관찰불동강도유양내력훈련후적골격기PPARα／δ표체、상관매활성、기섬유류형화운동능력적변화，탐구PPARα／δ재제고C57BL／6소서골격기유양내력중적작용이급훈련강도적영향。선용6주령웅성C57BL／6소서45지，수궤분위3개실험조，분별위76％VO2max 60min훈련조（LS）、86％VO2max60min훈련조（HS）화안정대조조（RC）。각운동조진행포태훈련，채용RT-PCR、Westernblot、MATPase염색검측골격기중PPARα／δ、CPT-1、BoAcDH화GPDH적표체급기섬유류형적변화。결과발현：운동각조골격기PPARα／δ전록화번역수평교대조조균유현저제고，이HS조균현저고우LS조；각운동조CPT-1、BoAc—DH적단백원체화운동능력교대조조균유현저제고，단운동조간몰유현저차이，GPDH적표체몰유현저개변；운동각조내력능력교대조조균유현저증강，단운동조간몰유현저차이；각조지간적골격기Ⅰ、Ⅱ형기섬유조성백분비균몰유현저차이。결론：PPARα／δ가능작위파점재골격기대내력훈련적괄응과정중발휘착중요작용，고강도훈련대우저충괄응과정적영향교대；운동병몰유도치골격기섬유류형발생현저변화。
Objective：From this view, the expression of PPARα and PPARδ, the activity of some interrelated enzymes （CPT-1, BoAcDH and GPDH）, the fiber types in skeletal muscles and the exercise capacity were studied on different exercise intensity of endurance training. Based on these studies, this paper discusses the role of PPARα/δ in skeletal muscle aerobiendurance enhancement of C57BL/6 mice and the effects of Different exercise intensity of endurance training on them. Method： Male C57BL/6 mice （6 weeks old） were randomly divided into three groups （two exercise groups and one control group）： medium and low intensity （12m/min）（LS） ,medium and high intensity （20m/min）（HS）, and no training （RC）. Every exercise group did treadmill training for six weeks. After each training program, the mice were sacrificed, and tissues were removed. RT-PCR, Western blot and mATPase staining were applied to analyze the effects of endurance training on skeletal muscle PPARα/δmRNA, PPARα/δ. CPT- 1 . BoAcDH 和 GPDH protein expression and their relationship with skeletal muscle types. Result：. The expression of PPARα/δ has an significant increase by Different exercise intensity of Endurance Training in every training group, and HS has an significant increase than LS, And endurance trainings induce the improvement of the enzyme activity and then a higher level of oxidation and metabolism in skeletal muscles, the expression of GPDH has no change, and training locomotory capacity has an significant increase. Aerobic exercise did not cause significant change in muscle fiber type implicate that skeletal muscle type switch may be affected by both cellular metabolism and some other unknown factors. Conclusion.PPARα/δ could play an important role on the adaption of skeletal muscle for endurance training as a target. This adaption is activated more easily under higher intensity. Aerobic exercise did not cause significant change in muscle fiber type.