心理学报
心理學報
심이학보
Acta Psychologica Sinica
2009年
1期
53~61
,共null页
沈悦娣 许百华 刘娜 魏丽丽 陈炜
瀋悅娣 許百華 劉娜 魏麗麗 陳煒
침열제 허백화 류나 위려려 진위
慢性应激 抑郁 神经肽Y 盐酸氟西汀 盐酸阿米替林
慢性應激 抑鬱 神經肽Y 鹽痠氟西汀 鹽痠阿米替林
만성응격 억욱 신경태Y 염산불서정 염산아미체림
depression; amitriptyline; chronic stress; fluoxetine; neuropeptide Y
神经肽Y（NPY）是一个多肽，广泛分布于周围和中枢神经系统，以下丘脑的浓度为高，参与机体神经内分泌、情绪、行为等的调节。为探讨慢性应激诱导的抑郁模型小鼠脑内NPY的表达，以及抗抑郁药的作用机理，采用慢性应激与孤养方法，建立抑郁小鼠模型。用旷场行为（Open—Field）法，观察模型组与正常对照组行为学改变方面的差异。将36只抑郁模型小鼠随机分成盐酸氟西汀组、盐酸阿米替林组、生理盐水治疗组，并与12只正常对照组比较。用半定量逆转录一聚合酶联反应（RT—PCR）法、细胞酶联免疫法及蛋白免疫印迹（Western-blot）法、免疫组织化学法，比较四组小鼠下丘脑NPY的表达。结果表明：（1）与正常组比较，抑郁模型组小鼠活动总路程与活动次数减少（P〈0．01），体重增速下降（p〈0．05）。（2）与正常组比较，抑郁模型组小鼠下丘脑NPY的mRNA表达下降（P〈0．01）；经盐酸氟西汀、盐酸阿米替林治疗后，下丘脑NPY的mRNA及蛋白表达增高，与生理盐水组比较差异显著（P〈0．01）。（3）与正常组比较，生理盐水治疗组胞膜和胞质呈棕黄色染色，显示出明显的免疫组化阳性反应；经盐酸氟西汀、盐酸阿米替林治疗后，胞膜和胞质的着色与背景色接近，免疫组化呈弱阳性、阴性反应。提示抑郁模型组小鼠脑内NPY表达水平有显著下降，使用抗抑郁药有利于提高脑内NPY的mRNA及蛋白表达，可能是此类药物产生抗抑郁效应的机制之一。
神經肽Y（NPY）是一箇多肽，廣汎分佈于週圍和中樞神經繫統，以下丘腦的濃度為高，參與機體神經內分泌、情緒、行為等的調節。為探討慢性應激誘導的抑鬱模型小鼠腦內NPY的錶達，以及抗抑鬱藥的作用機理，採用慢性應激與孤養方法，建立抑鬱小鼠模型。用曠場行為（Open—Field）法，觀察模型組與正常對照組行為學改變方麵的差異。將36隻抑鬱模型小鼠隨機分成鹽痠氟西汀組、鹽痠阿米替林組、生理鹽水治療組，併與12隻正常對照組比較。用半定量逆轉錄一聚閤酶聯反應（RT—PCR）法、細胞酶聯免疫法及蛋白免疫印跡（Western-blot）法、免疫組織化學法，比較四組小鼠下丘腦NPY的錶達。結果錶明：（1）與正常組比較，抑鬱模型組小鼠活動總路程與活動次數減少（P〈0．01），體重增速下降（p〈0．05）。（2）與正常組比較，抑鬱模型組小鼠下丘腦NPY的mRNA錶達下降（P〈0．01）；經鹽痠氟西汀、鹽痠阿米替林治療後，下丘腦NPY的mRNA及蛋白錶達增高，與生理鹽水組比較差異顯著（P〈0．01）。（3）與正常組比較，生理鹽水治療組胞膜和胞質呈棕黃色染色，顯示齣明顯的免疫組化暘性反應；經鹽痠氟西汀、鹽痠阿米替林治療後，胞膜和胞質的著色與揹景色接近，免疫組化呈弱暘性、陰性反應。提示抑鬱模型組小鼠腦內NPY錶達水平有顯著下降，使用抗抑鬱藥有利于提高腦內NPY的mRNA及蛋白錶達，可能是此類藥物產生抗抑鬱效應的機製之一。
신경태Y（NPY）시일개다태，엄범분포우주위화중추신경계통，이하구뇌적농도위고，삼여궤체신경내분비、정서、행위등적조절。위탐토만성응격유도적억욱모형소서뇌내NPY적표체，이급항억욱약적작용궤리，채용만성응격여고양방법，건립억욱소서모형。용광장행위（Open—Field）법，관찰모형조여정상대조조행위학개변방면적차이。장36지억욱모형소서수궤분성염산불서정조、염산아미체림조、생리염수치료조，병여12지정상대조조비교。용반정량역전록일취합매련반응（RT—PCR）법、세포매련면역법급단백면역인적（Western-blot）법、면역조직화학법，비교사조소서하구뇌NPY적표체。결과표명：（1）여정상조비교，억욱모형조소서활동총로정여활동차수감소（P〈0．01），체중증속하강（p〈0．05）。（2）여정상조비교，억욱모형조소서하구뇌NPY적mRNA표체하강（P〈0．01）；경염산불서정、염산아미체림치료후，하구뇌NPY적mRNA급단백표체증고，여생리염수조비교차이현저（P〈0．01）。（3）여정상조비교，생리염수치료조포막화포질정종황색염색，현시출명현적면역조화양성반응；경염산불서정、염산아미체림치료후，포막화포질적착색여배경색접근，면역조화정약양성、음성반응。제시억욱모형조소서뇌내NPY표체수평유현저하강，사용항억욱약유리우제고뇌내NPY적mRNA급단백표체，가능시차류약물산생항억욱효응적궤제지일。
Neuropeptide Y （NPY）, a conservative neuroendocrine peptides widely expressed in Peripheral and Central nervous system, is associated with depression, anxiety, and digestive disease. To study NPY expression in rat model of depression and to explore the mechanisms of the antidepressants, we developed a rat model of depression by the chronic stress exposure and isolation housing. We randomly divided 36 depressed rats into three groups （n = 12） and treated them with fluoxetine-, amitriptyline- and normal saline, respectively. Behavioral changes in these rats were analyzed in an Open-Field paradigm, and compared to a control group of 12 normal rats. The expression patterns of NPY in the hypothalamus were analyzed using Reverse Transcription - Polymerase Chain Reaction （RT-PCR）, Western Blot, and the Immunohistochemical assays. We made the following observations： （1） Compared with the normal rats, total traveling distance and activity frequency were reduced （p 〈 0.01 ） in the depressed rats; their weight gains were smaller （p 〈0 . 05） ; and their NPY mRNA levels in the hypothalamus were also lower （p 〈 0.01 ） ; （2） after treatment with either fluoxetine or amytriptyline, NPY mRNA and protein levels increased in brain of depressed rats. This difference is significance when compared to the normal saline group （p 〈 0.01 ）. ; （ 3 ） Compared with the normal control group, cell membrane and cytoplasm were stained to brown or yellow in the normal saline-treated group, indicating a positive immunohistochemical reaction. In contrast, the cell membrane and cytoplasm were stained to a color similar to the background color in the fluoxetine- and amytriptyline-treated groups, indicating a weak or negative immunohistochemical reaction. These results suggest that the brain NPY expression is reduced in the brain of depressed rats, and that a possible mechanism of the antidepressants is via enhancing rat brain NPY expression.
