CAJ | 학술논문

64只经过筛选能适应高强度训练的SD雄性大鼠随机分成5组：常氧安静组（C），低氧有氧训练组（A），常氧大强度训练组（D），高住低练组（H），低住高练组（L）。A组采用在低氧环境下低强度训练，D组、H组、L组采用间歇性高强度训练。D在常氧下训练居住，H组在常氧下训练在低氧下居住，L组在低氧下训练，在常氧下居住。A、D、H、L四组动物经过2周的适应性训练和5周的正式训练。在动物最后一次训练后24小时取动物的股四头肌浅层白肌，测试肌肉CK、ALD、PK、LDH、SDH的酶活性，测试肌肉糖原含量，提取肌肉的mRNA，通过RT-PCR检测各组HI-1α mRNA表达量。结果显示肌肉CK、ALD、LDH的活性各组间差异没有显著性（p〉0．05），PK、SDH和糖原各组间差异很显著（p〈0．01）。PK和糖原的变化趋势接近，C组和A组之间差异不显著且低于其他各组，H组的指标高于其他组且差异有显著性。H组的SDH显著低于A、D、L组，甚至低于C组，但与C组的差异没有显著性。A组的SDH显著高于其他各组。HIF-1α mRNA的RT—PCR测试结果与PK活性的变化趋势并不一致，L组的OID比值显著高于H组，C、A、D组则没有表达。结论：1．高强度间歇性低氧训练可以提高无氧糖酵解关键酶的活性，但对于磷酸原系统和三羧酸循环代谢酶的作用较弱。2．代谢酶的变化与不同的低氧训练方法相对应。3．糖酵解代谢酶的适应性变化受训练强度和训练环境的多重影响。4．HIF-1α的表达与缺氧刺激的程度有比较密切的关系5．高住低练的训练方法可能更适合于低氧高强度训练。
64지경과사선능괄응고강도훈련적SD웅성대서수궤분성5조：상양안정조（C），저양유양훈련조（A），상양대강도훈련조（D），고주저련조（H），저주고련조（L）。A조채용재저양배경하저강도훈련，D조、H조、L조채용간헐성고강도훈련。D재상양하훈련거주，H조재상양하훈련재저양하거주，L조재저양하훈련，재상양하거주。A、D、H、L사조동물경과2주적괄응성훈련화5주적정식훈련。재동물최후일차훈련후24소시취동물적고사두기천층백기，측시기육CK、ALD、PK、LDH、SDH적매활성，측시기육당원함량，제취기육적mRNA，통과RT-PCR검측각조HI-1α mRNA표체량。결과현시기육CK、ALD、LDH적활성각조간차이몰유현저성（p〉0．05），PK、SDH화당원각조간차이흔현저（p〈0．01）。PK화당원적변화추세접근，C조화A조지간차이불현저차저우기타각조，H조적지표고우기타조차차이유현저성。H조적SDH현저저우A、D、L조，심지저우C조，단여C조적차이몰유현저성。A조적SDH현저고우기타각조。HIF-1α mRNA적RT—PCR측시결과여PK활성적변화추세병불일치，L조적OID비치현저고우H조，C、A、D조칙몰유표체。결론：1．고강도간헐성저양훈련가이제고무양당효해관건매적활성，단대우린산원계통화삼최산순배대사매적작용교약。2．대사매적변화여불동적저양훈련방법상대응。3．당효해대사매적괄응성변화수훈련강도화훈련배경적다중영향。4．HIF-1α적표체여결양자격적정도유비교밀절적관계5．고주저련적훈련방법가능경괄합우저양고강도훈련。
Objects and Methods： 64 male rats, which were selected and can adopted to intense training, were randomly assigned to one of five groups： normoxic sedentary group （C）, hypoxia aerobic endurance exercise group （A）, normoxic intense exercise group （D）, living--high train-- low group（H）, living--low train--high group（L） . Group A were trained with treadmill at 16 % O2. Group D, H, L were trained with intense intermittent train. Group D lived and trained in normoxic; Group H trained in normoxic and lived in hypoxia, Group L trained in hypoxia and lived in normoxic. Quadriceps femoris superficial layer（white muscle） were get after 24h of last training. The activity of CK, ALD, PK, LDH, SDH and glyconen concentration were detected by test kit. The expression of HIF--1α mRNA was detected by RT--PCR. Results： The difference of CK, ALD, LDH activity among 5 groups had no significant （p〉0. 05 ）, but there were significant difference of PK, SDH activity and glyconen concentration in animals of all groups. The tendency of PK and glyconen was similar. Group C and A were lower than other groups, and difference between A and C had no significant. Group H higher than any other groups（p〈0. 05 ）. SDH activity of group H lower than A,D,L（p〈0. 05）,even lower than C （NS）. The result of RT--PCR show that the HIF--1α gene expression of group L higher than H, and there were no gene expression of group C, A, D. Conclusions： 1. Intense intermittent hypoxia training can enhance the key enzyme activity of glycolysis, but had less effect on metabolism enzyme activity of ATP--CP system and Krebs cycle. 2. Transformations of metabolism enzyme responded to the hypoxia training protocol. 3. The adaptations of glycolysis enzymes were influenced by multiplex factor, i.e. intensity of training and hypoxia stimulate. 4. Gene expression of HIF--1α had close relationship with the level of hypoxia stimulate. 5. Living--high training--low may be more suit to hypoxia intense training.