周口师范学院学报
週口師範學院學報
주구사범학원학보
Journal of Zhoukou Normal University
2009年
2期
71~72
,共null页
裴朝玉 方国波 谢红 杨秀 林开春
裴朝玉 方國波 謝紅 楊秀 林開春
배조옥 방국파 사홍 양수 림개춘
神农霉素Ⅱ号组分 定量分析 高效液相 提取效率 相关性
神農黴素Ⅱ號組分 定量分析 高效液相 提取效率 相關性
신농매소Ⅱ호조분 정량분석 고효액상 제취효솔 상관성
Shennongmycin Ⅱ; quantity analysis ; HPLC; extractal effect ; relation
对神农霉素Ⅱ号组分进行了HPLC定量分析方法、溶剂提取效率及HPLC法与生物法测定结果相关性的研究.结果表明,水饱和正丁醇一次提取效率较高,为86.03%.用HPLC法分析时,标准品的浓度在21.2~846.0μg/mL内分析结果有较好的线性关系.其相关系数、相对标准偏差、回收率分别为0.9995,0.1080%,97.60%~100.23%.HPLC法与生物法测定结果相关系数为0.9603,表明生物法和HPLC法测定结果有较好的相关性.
對神農黴素Ⅱ號組分進行瞭HPLC定量分析方法、溶劑提取效率及HPLC法與生物法測定結果相關性的研究.結果錶明,水飽和正丁醇一次提取效率較高,為86.03%.用HPLC法分析時,標準品的濃度在21.2~846.0μg/mL內分析結果有較好的線性關繫.其相關繫數、相對標準偏差、迴收率分彆為0.9995,0.1080%,97.60%~100.23%.HPLC法與生物法測定結果相關繫數為0.9603,錶明生物法和HPLC法測定結果有較好的相關性.
대신농매소Ⅱ호조분진행료HPLC정량분석방법、용제제취효솔급HPLC법여생물법측정결과상관성적연구.결과표명,수포화정정순일차제취효솔교고,위86.03%.용HPLC법분석시,표준품적농도재21.2~846.0μg/mL내분석결과유교호적선성관계.기상관계수、상대표준편차、회수솔분별위0.9995,0.1080%,97.60%~100.23%.HPLC법여생물법측정결과상관계수위0.9603,표명생물법화HPLC법측정결과유교호적상관성.
The article was based on the quantity analysis to Shennongmycin Ⅱ by HPLC,the extractal potencyion of nbutyl alcohol to Shennongmycin Ⅱ and the relation between bio - potency and chemical potency to Shennongmycin Ⅱ. The first extractal potencyion of n - butyl alcohol to Shennongmycin Ⅱ is 86.03%. The results of the quantity analysis showed that the correlation coefficient,variability coefficient, RSD to Shennongmycin Ⅱ are 0. 999 5,0. 108 0%,97.60% 100.23 % respectively. The correlation coefficient between bio-potency and chemical potency to Shennongmycin Ⅱ is 0. 9603.
