中国体育科技
中國體育科技
중국체육과기
China Sport Science and Technology
2009年
3期
137~140
,共null页
席翼 温含 武雅琼 苏丽 姜宁 牛燕媚 苑红 傅力
席翼 溫含 武雅瓊 囌麗 薑寧 牛燕媚 苑紅 傅力
석익 온함 무아경 소려 강저 우연미 원홍 부력
急性有氧运动 PPARδ mRNA和蛋白表达 酶活性 鼠 动物实验
急性有氧運動 PPARδ mRNA和蛋白錶達 酶活性 鼠 動物實驗
급성유양운동 PPARδ mRNA화단백표체 매활성 서 동물실험
acute aerobic exercise ; PPARS ; expression of rnRNA and protein ; activity of enzyme ; mice ; animal experiment
目的：观察急性有氧运动对PPARδ表达和相关代谢酶活性的影响，以研讨耐力训练使骨骼肌有氧代谢能力产生适应性变化的机制。方法：6周龄雄性C57BL／6小鼠，体重为15±2g，对照组8只，运动组32只。运动组以20m／min速度在跑台上运动120min，并在运动后的即刻、1h、3h和6h分别处死8只小鼠。采用RT-PCR和Westem blot方法检测骨骼肌中的PPARδ、CPT-1和BoAcDH的表达。结果：与对照组相比，运动组PPARδ的mRNA和蛋白表达量分别增加了2．3倍和1．0倍（P〈O．05），CPT-1和BoAcDH的蛋白表达分别提高了1．3倍和1．9倍。结论：急性有氧运动诱导了PPARδ的过量表达，从而调控脂肪酸氧化相关酶的活性，促进骨骼肌有氧代谢能力增强，成为长期训练使肌肉产生适应性变化的基础。
目的：觀察急性有氧運動對PPARδ錶達和相關代謝酶活性的影響，以研討耐力訓練使骨骼肌有氧代謝能力產生適應性變化的機製。方法：6週齡雄性C57BL／6小鼠，體重為15±2g，對照組8隻，運動組32隻。運動組以20m／min速度在跑檯上運動120min，併在運動後的即刻、1h、3h和6h分彆處死8隻小鼠。採用RT-PCR和Westem blot方法檢測骨骼肌中的PPARδ、CPT-1和BoAcDH的錶達。結果：與對照組相比，運動組PPARδ的mRNA和蛋白錶達量分彆增加瞭2．3倍和1．0倍（P〈O．05），CPT-1和BoAcDH的蛋白錶達分彆提高瞭1．3倍和1．9倍。結論：急性有氧運動誘導瞭PPARδ的過量錶達，從而調控脂肪痠氧化相關酶的活性，促進骨骼肌有氧代謝能力增彊，成為長期訓練使肌肉產生適應性變化的基礎。
목적：관찰급성유양운동대PPARδ표체화상관대사매활성적영향，이연토내력훈련사골격기유양대사능력산생괄응성변화적궤제。방법：6주령웅성C57BL／6소서，체중위15±2g，대조조8지，운동조32지。운동조이20m／min속도재포태상운동120min，병재운동후적즉각、1h、3h화6h분별처사8지소서。채용RT-PCR화Westem blot방법검측골격기중적PPARδ、CPT-1화BoAcDH적표체。결과：여대조조상비，운동조PPARδ적mRNA화단백표체량분별증가료2．3배화1．0배（P〈O．05），CPT-1화BoAcDH적단백표체분별제고료1．3배화1．9배。결론：급성유양운동유도료PPARδ적과량표체，종이조공지방산양화상관매적활성，촉진골격기유양대사능력증강，성위장기훈련사기육산생괄응성변화적기출。
Objective： Observing the influence of acute aerobic exercise on the expression of PPARδ and activity of some interrelated enzymes in order to study the mechanism of aerobics causing skeletal muscle biologically adaptive change. Method： male mice （C57BL/6）, 6-week- old, 15±2g in weight, 8 in control group and 32 in exercise group. The mice in exercise group run 120mins at a speed of 20m/min, and they were killed immediately, an hour, three hours and six hours after exercises respectively. RT-PCR and Western blot are applied to examine the expression of PPARδ, CPT-1 and BoAcDH in skeletal muscles. Results： In comparison with control group, the expression of mRNA and protein in PPARδ has increased respectively by 2. 3 times and 1 time（P〈0.05）in the exercise group, and the expression of protein in CPT- land BoAcDH has increased respectively by 1.3 and 1.9 times. Conclusion： Acute aerobic exercise induced the excessive expression of PPARδ, thus adjusted the activity of interrelated enzyme in oxidizing fatty acid and enhanced the increase in the ability of aerobic metabolism to become the change basis of adaptability caused by long-term training.
