天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2009年
3期
261~264
,共null页
递增负荷运动 心肌细胞 凋亡 Bcl-2 Bax
遞增負荷運動 心肌細胞 凋亡 Bcl-2 Bax
체증부하운동 심기세포 조망 Bcl-2 Bax
incremental exercise; cardiocyte; apoptosis; Bcl-2; Bax
目的：研究递增负荷运动对大鼠心肌细胞凋亡调控基因Bcl-2和Bax的影响，探讨递增负荷运动对心肌细胞的作用。方法：将大鼠分为0Wk～5Wk运动组，每组又分为：安静组、运动后即刻组、运动后3h组和运动后24h组，负荷从20m／min递增到40m／min，采用反转录聚合酶链式反应（RT—PCR）技术，观察其基因的表达。结果：（1）各周运动后即刻，Bcl-2mRNA表达0Wk较1Wk、3Wk和5Wk显著升高，Bax表达5Wk较4Wk显著升高；（2）各周运动后3h，Bcl-2表达4Wk较2Wk极显著降低，Bax表达2Wk和5Wk分别较0Wk显著升高和降低；（3）各周运动后24h，Bcl-2表达各周都较OWk有极显著性降低，Bax表达5Wk较0Wk和4Wk明显降低。结论：递增负荷运动后即刻，心肌表现出一种自我保护性的应激反应，运动后3h与24h，尤其是24h，心肌抗凋亡能力减弱。
目的：研究遞增負荷運動對大鼠心肌細胞凋亡調控基因Bcl-2和Bax的影響，探討遞增負荷運動對心肌細胞的作用。方法：將大鼠分為0Wk～5Wk運動組，每組又分為：安靜組、運動後即刻組、運動後3h組和運動後24h組，負荷從20m／min遞增到40m／min，採用反轉錄聚閤酶鏈式反應（RT—PCR）技術，觀察其基因的錶達。結果：（1）各週運動後即刻，Bcl-2mRNA錶達0Wk較1Wk、3Wk和5Wk顯著升高，Bax錶達5Wk較4Wk顯著升高；（2）各週運動後3h，Bcl-2錶達4Wk較2Wk極顯著降低，Bax錶達2Wk和5Wk分彆較0Wk顯著升高和降低；（3）各週運動後24h，Bcl-2錶達各週都較OWk有極顯著性降低，Bax錶達5Wk較0Wk和4Wk明顯降低。結論：遞增負荷運動後即刻，心肌錶現齣一種自我保護性的應激反應，運動後3h與24h，尤其是24h，心肌抗凋亡能力減弱。
목적：연구체증부하운동대대서심기세포조망조공기인Bcl-2화Bax적영향，탐토체증부하운동대심기세포적작용。방법：장대서분위0Wk～5Wk운동조，매조우분위：안정조、운동후즉각조、운동후3h조화운동후24h조，부하종20m／min체증도40m／min，채용반전록취합매련식반응（RT—PCR）기술，관찰기기인적표체。결과：（1）각주운동후즉각，Bcl-2mRNA표체0Wk교1Wk、3Wk화5Wk현저승고，Bax표체5Wk교4Wk현저승고；（2）각주운동후3h，Bcl-2표체4Wk교2Wk겁현저강저，Bax표체2Wk화5Wk분별교0Wk현저승고화강저；（3）각주운동후24h，Bcl-2표체각주도교OWk유겁현저성강저，Bax표체5Wk교0Wk화4Wk명현강저。결론：체증부하운동후즉각，심기표현출일충자아보호성적응격반응，운동후3h여24h，우기시24h，심기항조망능력감약。
Objective： This paper probed the effects of incremental exercise on apoptotic gene Bcl-2 and Bax in myocardiumin order to study the regulating mechanism of incremental exercise on apoptotic gene Bcl-2, Bax in myocardiumin. Method： The rats were divided into quiet group, immediate after exercise, 3h after exercise and 24h after exercise. The expression of genes Bcl-2, Bax and the Bcl-2/Bax ratio were recorded in sedentary state, immediately af- ter training and 3h and 24h after training were determined by RT-PCR. Results： （1）The expression of gene Bcl-2 determined immediately after training was higher in 1Wk and 5Wk as compared with that in 0Wk. The expression of gene Bax was higher in 5Wk than that in 4Wk. （2）At 3h after training the expression of gene Bcl-2 was significantly reduced in 4Wk as compared with that in 2Wk. The expression of gene Bax was lower in 0Wk as compared with that in 2Wk, also lower than that in 5Wk. （3）During 0Wk-SWk, at 24h after training the expression of gene Bcl-2 was the lowest in 0Wk. The expression of gene Bax was decreased in 5Wk as compared with that in 0Wk and 4Wk. Conclusion： The cardiomyocyte produced an active response to self-protect immediately after training. The anti-apoptosis ability of cardiomyocyte went down at 3h and 24h after training, especially at 24h.
