体育科学
體育科學
체육과학
China Sport Science
2009年
7期
65~70
,共null页
刘双虎 胡扬 衣龙彦 吉洪书 王景玲 聂晶
劉雙虎 鬍颺 衣龍彥 吉洪書 王景玲 聶晶
류쌍호 호양 의룡언 길홍서 왕경령 섭정
IGFBP3 基因多态性 低氧训练 左心室功能 分子标记
IGFBP3 基因多態性 低氧訓練 左心室功能 分子標記
IGFBP3 기인다태성 저양훈련 좌심실공능 분자표기
IGFBP3 ; genetic polymorphism; HiHiLo; left ventricular function ; molecularmaker
目的：通过胰岛素样生长因子结合蛋白3（IGFBP3）基因多态性与低氧训练一高住高练低训（HiHiLo）后左心室功能训练效果的关联性分析，为预测低氧训练效果，制定个性化低氧训练方案提供分子标记。方法：68名男子大学生运动员接受为期4周HiHiLo训练，使用Doppler超声心动仪测试其训练前、后安静状态和递增负荷运动下的心功能指标。采用PCR-RFLP方法解析受试者IGFBP3基因A-202C、Pr0158His位点的多态性，并与训练前、后心功能指标进行关联分析。结果：A-202C位点多态性与50W／SV和CO、100w／SV和CO初始值关联，均为AA基因型高于AC基因型；与150w／HR、CI的训练效果关联，表现为AA基因型变化显著；Pro158His位点多态性与50W／EF、CO和CI，100w／CO初始值关联，均为AA基因型高于AC基因型，与安静状态／HR、50w／SI、100W／SI的训练效果关联，表现为AC基因型变化显著。结论：IGFBP3基因A-202C位点AA基因型和Pr0158His位点AC基因型对于4周HiHiLo训练在左心室功能方面表现出较好的敏感性，可作为预测低氧训练效果的分子标记。
目的：通過胰島素樣生長因子結閤蛋白3（IGFBP3）基因多態性與低氧訓練一高住高練低訓（HiHiLo）後左心室功能訓練效果的關聯性分析，為預測低氧訓練效果，製定箇性化低氧訓練方案提供分子標記。方法：68名男子大學生運動員接受為期4週HiHiLo訓練，使用Doppler超聲心動儀測試其訓練前、後安靜狀態和遞增負荷運動下的心功能指標。採用PCR-RFLP方法解析受試者IGFBP3基因A-202C、Pr0158His位點的多態性，併與訓練前、後心功能指標進行關聯分析。結果：A-202C位點多態性與50W／SV和CO、100w／SV和CO初始值關聯，均為AA基因型高于AC基因型；與150w／HR、CI的訓練效果關聯，錶現為AA基因型變化顯著；Pro158His位點多態性與50W／EF、CO和CI，100w／CO初始值關聯，均為AA基因型高于AC基因型，與安靜狀態／HR、50w／SI、100W／SI的訓練效果關聯，錶現為AC基因型變化顯著。結論：IGFBP3基因A-202C位點AA基因型和Pr0158His位點AC基因型對于4週HiHiLo訓練在左心室功能方麵錶現齣較好的敏感性，可作為預測低氧訓練效果的分子標記。
목적：통과이도소양생장인자결합단백3（IGFBP3）기인다태성여저양훈련일고주고련저훈（HiHiLo）후좌심실공능훈련효과적관련성분석，위예측저양훈련효과，제정개성화저양훈련방안제공분자표기。방법：68명남자대학생운동원접수위기4주HiHiLo훈련，사용Doppler초성심동의측시기훈련전、후안정상태화체증부하운동하적심공능지표。채용PCR-RFLP방법해석수시자IGFBP3기인A-202C、Pr0158His위점적다태성，병여훈련전、후심공능지표진행관련분석。결과：A-202C위점다태성여50W／SV화CO、100w／SV화CO초시치관련，균위AA기인형고우AC기인형；여150w／HR、CI적훈련효과관련，표현위AA기인형변화현저；Pro158His위점다태성여50W／EF、CO화CI，100w／CO초시치관련，균위AA기인형고우AC기인형，여안정상태／HR、50w／SI、100W／SI적훈련효과관련，표현위AC기인형변화현저。결론：IGFBP3기인A-202C위점AA기인형화Pr0158His위점AC기인형대우4주HiHiLo훈련재좌심실공능방면표현출교호적민감성，가작위예측저양훈련효과적분자표기。
The purpose of this study was to search for the possible association between poly- morphism of IGFBP3 gene and left ventricular function training efficiency after HiHiLo, thus predict the efficiency of HiHiLo and as a genetic marker for individual hypoxic training proto- col. 68 male college-student athletes underwent HiHiLo training for 4 weeks, left ventricular function under static as wel[ as incremental exercise status were respectively measured by Doppler echocardiogram before and after HiHiLo training. Single nuclear polymorphisms of A- 202C and Pro158His in IGFBP3 gene were genotyped by PCR-RFLP, and were associated with measured left ventricular function phenotypes. It was found that A-202C locus polymor- phism was associated with 50W/SV＆CO, 100W/SV＆CO at baseline, those who carried with AA genotype had higher level than that with AC genotype, and associated with training re- sponse in 150W/HR and CI, those who carried with AA genotype had more significant chan- ges. Pro158His locus polymorphism was associated with 50W/EF＆CO＆CI, 100W/CO at baseline, the subjects with AA genotype had higher level than AC genotype, and associated with HiHiLo training response in Static/HR, 50W/SI and 100W/SI, the subjects with AC genotype had more significant changes. It concludes that APt genotype of A-202C and AC gen- otype of Pro158His may be more sensitive to effect of HiHiLo on left ventricular function and will be a molecular marker for predicting effect of hypoxic training.
