CAJ | 학술논문

以水解度为优化的指标，利用单因素试验研究酶解温度、pH值、反应时间等因素对鱼蛋白水解的影响，利用中心组合试验方法优化酶的添加量和蛋白质浓度．结果表明：复合蛋白酶水解罗非鱼的最佳条件为：加酶量17660U／g蛋白、蛋白质质量分数2．50％、水解温度55℃、pH值7．5、水解时间10h，在此条件下，水解体系的氨基酸态氮含量达到了1．41mg／mL，水解度为35．25％，挥发性盐基氮含量为0．048mg／mL，得到用复合蛋白酶水解罗非鱼的最佳工艺条件．
이수해도위우화적지표，이용단인소시험연구매해온도、pH치、반응시간등인소대어단백수해적영향，이용중심조합시험방법우화매적첨가량화단백질농도．결과표명：복합단백매수해라비어적최가조건위：가매량17660U／g단백、단백질질량분수2．50％、수해온도55℃、pH치7．5、수해시간10h，재차조건하，수해체계적안기산태담함량체도료1．41mg／mL，수해도위35．25％，휘발성염기담함량위0．048mg／mL，득도용복합단백매수해라비어적최가공예조건．
The aim of this paper is to develop a process for hydrolysis Tilapia with protamex protease. The effects of the main parameters including hydrolysis temperature, pH value, hydrolysis time, were investigated with single factor experiment, and dosage of enzyme, protein concentration were investigated with response surface methodology （RSM）. The optimized conditions are：concentration of protamex protease of 17 660 U/g protein, protein concentration of 2.50%, hydrolysis temperature of 55 ℃ ,hydrolysis pH value of 7. 5, hydrolysis time of 10 hours. Under these conditions, amino acid nitrogen concentration attains 1.41 mg/mL, hydrolysis degree attains 35.25 %, and total volatile basic nitrogen reachs 0. 048 mg/mL.