周口师范学院学报
週口師範學院學報
주구사범학원학보
Journal of Zhoukou Normal University
2009年
5期
81~82
,共null页
细胞保存液 0.85%NaCl 琼脂糖凝胶电泳
細胞保存液 0.85%NaCl 瓊脂糖凝膠電泳
세포보존액 0.85%NaCl 경지당응효전영
cell preservation liquid; 0.85% NaCl; agarose gelelectrophoresis;
比较不同方法保存培养的人胃癌SGC7901细胞,用QIAGEN DNA提取试剂盒提取DNA,琼脂糖凝胶电泳检测DNA提取效果,找到一种简便、经济的保存方法.结果显示,0.85%NaCl和细胞保存液保存5 d的效果好,而0.85%NaCl更经济实用.
比較不同方法保存培養的人胃癌SGC7901細胞,用QIAGEN DNA提取試劑盒提取DNA,瓊脂糖凝膠電泳檢測DNA提取效果,找到一種簡便、經濟的保存方法.結果顯示,0.85%NaCl和細胞保存液保存5 d的效果好,而0.85%NaCl更經濟實用.
비교불동방법보존배양적인위암SGC7901세포,용QIAGEN DNA제취시제합제취DNA,경지당응효전영검측DNA제취효과,조도일충간편、경제적보존방법.결과현시,0.85%NaCl화세포보존액보존5 d적효과호,이0.85%NaCl경경제실용.
Compared the different preservation of DNA in the cultured human gastric cancer cells（SGC7901）.To find an easy and economical way,we extracted DNA from the the different preservations using reagent kit of QIAGEN,then tested the integrality of the genome through agarose gelelectrophoresis.The results showed that 0.85% NaCl and cell preservation liquid can preserve for five days,but 0.85% NaCl more economical.
