体育科学
體育科學
체육과학
China Sport Science
2010年
1期
66~69
,共null页
BrdU NeuN 神经发生 海马 鼠 动物实验
BrdU NeuN 神經髮生 海馬 鼠 動物實驗
BrdU NeuN 신경발생 해마 서 동물실험
BrdU; NeuN; neurogegesis; hippocampus; rat; animal experiment;
目的：探讨运动频次和隔周递增强度运动对大鼠海马神经发生的影响。方法：将60只雄性5周龄SD大鼠随机分为安静对照（C）组、每天运动（E0）组、隔1日运动（E1）组、隔3日运动（E3）组、隔周运动（E7）组和隔周递增强度运动（E7I）组。运动方式为跑台运动。腹腔注射BrdU于各组大鼠,持续注射6天。采用免疫组织荧光染色技术,检测各组大鼠海马齿状回内新生细胞和新生神经元数量。结果：各组动物海马齿状回内BrdU免疫阳性（Br-dU＋）细胞数目为：C组37.62±9.02,E0组46.01±10.82,E1组49.06±12.07,E3组41.20±11.02,E7组37.23±16.45,E7I组53.97±10.67。各组大鼠海马齿状回内BrdU和NeuN免疫双阳性（BrdU＋＋NeuN＋）细胞数分别为：C组15.26±4.42;E0组17.61±3.86,E1组21.93±5.60,E3组16.28±5.37,E7组15.80±7.50,E7I组24.99±5.44。结论：在间隔不同时间的小强度运动中,隔日小强度运动最有利于大鼠海马齿状回神经发生;隔周递增运动强度也有利于大鼠海马齿状回神经发生。
目的：探討運動頻次和隔週遞增彊度運動對大鼠海馬神經髮生的影響。方法：將60隻雄性5週齡SD大鼠隨機分為安靜對照（C）組、每天運動（E0）組、隔1日運動（E1）組、隔3日運動（E3）組、隔週運動（E7）組和隔週遞增彊度運動（E7I）組。運動方式為跑檯運動。腹腔註射BrdU于各組大鼠,持續註射6天。採用免疫組織熒光染色技術,檢測各組大鼠海馬齒狀迴內新生細胞和新生神經元數量。結果：各組動物海馬齒狀迴內BrdU免疫暘性（Br-dU＋）細胞數目為：C組37.62±9.02,E0組46.01±10.82,E1組49.06±12.07,E3組41.20±11.02,E7組37.23±16.45,E7I組53.97±10.67。各組大鼠海馬齒狀迴內BrdU和NeuN免疫雙暘性（BrdU＋＋NeuN＋）細胞數分彆為：C組15.26±4.42;E0組17.61±3.86,E1組21.93±5.60,E3組16.28±5.37,E7組15.80±7.50,E7I組24.99±5.44。結論：在間隔不同時間的小彊度運動中,隔日小彊度運動最有利于大鼠海馬齒狀迴神經髮生;隔週遞增運動彊度也有利于大鼠海馬齒狀迴神經髮生。
목적：탐토운동빈차화격주체증강도운동대대서해마신경발생적영향。방법：장60지웅성5주령SD대서수궤분위안정대조（C）조、매천운동（E0）조、격1일운동（E1）조、격3일운동（E3）조、격주운동（E7）조화격주체증강도운동（E7I）조。운동방식위포태운동。복강주사BrdU우각조대서,지속주사6천。채용면역조직형광염색기술,검측각조대서해마치상회내신생세포화신생신경원수량。결과：각조동물해마치상회내BrdU면역양성（Br-dU＋）세포수목위：C조37.62±9.02,E0조46.01±10.82,E1조49.06±12.07,E3조41.20±11.02,E7조37.23±16.45,E7I조53.97±10.67。각조대서해마치상회내BrdU화NeuN면역쌍양성（BrdU＋＋NeuN＋）세포수분별위：C조15.26±4.42;E0조17.61±3.86,E1조21.93±5.60,E3조16.28±5.37,E7조15.80±7.50,E7I조24.99±5.44。결론：재간격불동시간적소강도운동중,격일소강도운동최유리우대서해마치상회신경발생;격주체증운동강도야유리우대서해마치상회신경발생。
Objective： To investigate effects of movement frequency and incremental exercise intensity on hippocampal neurogenesis in rats. Methods.. Sixty male SD rats of 5-week-old were randomly divided into 6 groups ： The sedentary control （C） group, the everyday exercise （E0） group, the every other day exercise （ E1 ） group, the every three days exercise （ E3 ） group, the every other week exercise （E7）group and the every other week increasing intensity exercise （E7I） group. All rats except C group were forced to run on treadmill. Immunofluoreseence staining was used to evaluate the level of neurogenesis. Results：The number of BrdU positive cells in dentate gyrus of hippocampus was 37. 62±9.02 in C group,46.01± 10. 82 in E0 group,49.06 ± 12. 07 in E1 group, 41. 20 ± 11. 02 in E3 group, 37. 23 ± 16. 45 in E7 group, 53.97 ± 10.67 in E7I group, respectively. Conclusions ： Among all small intensity exercises which have different exercise frequency, the every other day exercise is most benefit of hippocampal neurogenesis in SD rats, the every other week increasing intensity exercise also is benefit of hip~pal neurogenesis in SD rats.
