CAJ | 학술논문

本文详细描述了一种全自动化的行为检测方案。在自然饲养环境／检测环境（24／7）中，我们测量了小鼠针对两个给食器中获得食物的比例与它们在相应给食器停留时长的比例进行匹配的精确性与准确性。该方案是对传统条件性习得（trials-to-acquisition）行为测验设备的改进，可以检测动物时间间隔能力的精确性与准确性，对定时目标选择的相关概率的效果，以及记忆一天中从不同给食器中获得食物次数的精确性与准确性。该压缩系统避免了在整个实验过程中对小鼠的持握操作，可忽略实验者／技术员的实验操作时间，而且可以递送小鼠置入实验环境后，7-9个实验日中全部3组实验流程产生的大量结果。其中，第一个实验流程为单个24小时周期内完成的时间匹配能力的筛查，它对动物的时间、空间估计能力的记忆机制进行精确检测。因此，该系统允许在有限的实验空间、较短的实验周期内，对大量的实验小鼠进行有可能存在的学习记忆能力缺陷进行大规模筛查。此外，该系统运行所依赖的软件可以在公共开放平台获得。
본문상세묘술료일충전자동화적행위검측방안。재자연사양배경／검측배경（24／7）중，아문측량료소서침대량개급식기중획득식물적비례여타문재상응급식기정류시장적비례진행필배적정학성여준학성。해방안시대전통조건성습득（trials-to-acquisition）행위측험설비적개진，가이검측동물시간간격능력적정학성여준학성，대정시목표선택적상관개솔적효과，이급기억일천중종불동급식기중획득식물차수적정학성여준학성。해압축계통피면료재정개실험과정중대소서적지악조작，가홀략실험자／기술원적실험조작시간，이차가이체송소서치입실험배경후，7-9개실험일중전부3조실험류정산생적대량결과。기중，제일개실험류정위단개24소시주기내완성적시간필배능력적사사，타대동물적시간、공간고계능력적기억궤제진행정학검측。인차，해계통윤허재유한적실험공간、교단적실험주기내，대대량적실험소서진행유가능존재적학습기억능력결함진행대규모사사。차외，해계통운행소의뢰적연건가이재공공개방평태획득。
We describe a fully automated, live-in 24/7 test environment, with experimental protocols that measure the accuracy and precision with which mice match the ratio of their expected visit durations to the ratio of the incomes obtained from two hoppers, the progress of instrumental and classical conditioning （trials-to-acquisition）, the accuracy and precision of interval timing, the effect of relative probability on the choice of a timed departure target, and the accuracy and precision of memory for the times of day at which food is available. The system is compact; it obviates the handling of the mice during testing; it requires negligible amounts of experimenter/technician time; and it delivers clear and extensive results from 3 protocols within a total of 7-9 days after the mice are placed in the test environment. Only a single 24-hour period is required for the completion of first protocol （the matching protocol）, which is strong test of temporal and spatial estimation and memory mechanisms. Thus, the system permits the extensive screening of many mice in a short period of time and in limited space. The software is publicly available.