CAJ | 학술논문

目的：研究耐力训练对AMPKα2基因敲除小鼠骨骼肌CPT-1mRNA和蛋白表达的影响，探讨耐力训练中AMPKα2调节脂肪酸代谢的可能途径。研究方法：两月龄C57BL／6J野生（WT）和AMPKα2基因敲除（KO）小鼠各20只，各自随机分为安静对照组，耐力训练组，每组10只。两训练组进行4周，每天1h，速度为12m／min的跑台训练，测定股四头叽CPT-1mRNA和蛋白表达以及血清FFA、TG水平。结果：4周耐力训练之后，WT鼠和KO鼠骨骼肌CPT—1mRNA和蛋白表达，与安静对照组相比均显著升高，且两训练组之间无显著差异。结论：耐力训练中AMPKα2不是CPT-1的唯一上游调节因子，可能有其他途径参与CPT-1表达的调节。
목적：연구내력훈련대AMPKα2기인고제소서골격기CPT-1mRNA화단백표체적영향，탐토내력훈련중AMPKα2조절지방산대사적가능도경。연구방법：량월령C57BL／6J야생（WT）화AMPKα2기인고제（KO）소서각20지，각자수궤분위안정대조조，내력훈련조，매조10지。량훈련조진행4주，매천1h，속도위12m／min적포태훈련，측정고사두교CPT-1mRNA화단백표체이급혈청FFA、TG수평。결과：4주내력훈련지후，WT서화KO서골격기CPT—1mRNA화단백표체，여안정대조조상비균현저승고，차량훈련조지간무현저차이。결론：내력훈련중AMPKα2불시CPT-1적유일상유조절인자，가능유기타도경삼여CPT-1표체적조절。
Objective： To study the effect of endurance training on skeletal muscle CPT-1 mRNA and protein expression of α2-AMPK whole-body knockout （KO） mice. Meanwhile, we also explore the possible role of AMPKα2 in regulating fatty acid oxidation in endurance training. Methods： C57BL/6J wild-type （WT） mice （n=20） and α2-AMPK whole-body knockout （KO） mice （n=20）, two months old, were randomly subdivided into control groups and exercise groups. Quadriceps femoris muscle was removed after 4 weeks tradmill running （12m/min, 1h/day）. Expression of CPT-1 mRNA and protein, blood serum FFA and TG levels were measured. Results： After 4 weeks endurance training, skeletal muscle CPT-1 mRNA and protein expression of WT and KO mice were significantly higher than control groups. At the same time, there were no significant differences between two exercise groups. Conclusion： AMPKα2 is not the only upstream regulatory factor of CPT-1 in endurance training, and there could have other factors which regulate CPT-1 mRNA and protein expression during this kind of training.