CAJ | 학술논문

目的：探讨运动对大鼠冠状动脉降钙素基因相关肽（CGRP）、内皮素（ET-1）和一氧化氮合酶（NOS）表达的影响。方法：健康雄性SD3月龄大鼠24只，分为安静对照组、小强度运动组和大强度运动组，运动组采用大鼠跑台运动方式，建立大鼠不同强度运动模型，运用免疫组织化学SABC法研究不同强度运动对大鼠冠状动脉CGRP、ET-1、神经型一氧化氮合酶（nNOS）、诱导型一氧化氮合酶（iNOS）和内皮型一氧化氮合酶（eNOS）表达的影响。结果：与安静对照组比较，小强度运动组和大强度运动组CGRP、nNOS、iNOS和eNOS均显著性升高，小强度运动组ET-1显著降低，大强度运动组ET-1显著性升高。与小强度运动组比较，大强度运动组CGRP、ET-1、nNOS、iNOS和eNOS的表达变化均有届著性差异。小强度运动组和大强度运动组ET-1／CGRP和ET-1／NOS比值均低于安静对照组。结论：运动可引起大鼠冠状动脉CGRP、ET-1及NOS的表达变化，且与运动强度关系密切。因此认为，不同强度运动引起ET—1／CGRP和ET-1／NOS比值的变化可能是运动引起心血管生物功能改变的因素之一。
목적：탐토운동대대서관상동맥강개소기인상관태（CGRP）、내피소（ET-1）화일양화담합매（NOS）표체적영향。방법：건강웅성SD3월령대서24지，분위안정대조조、소강도운동조화대강도운동조，운동조채용대서포태운동방식，건립대서불동강도운동모형，운용면역조직화학SABC법연구불동강도운동대대서관상동맥CGRP、ET-1、신경형일양화담합매（nNOS）、유도형일양화담합매（iNOS）화내피형일양화담합매（eNOS）표체적영향。결과：여안정대조조비교，소강도운동조화대강도운동조CGRP、nNOS、iNOS화eNOS균현저성승고，소강도운동조ET-1현저강저，대강도운동조ET-1현저성승고。여소강도운동조비교，대강도운동조CGRP、ET-1、nNOS、iNOS화eNOS적표체변화균유계저성차이。소강도운동조화대강도운동조ET-1／CGRP화ET-1／NOS비치균저우안정대조조。결론：운동가인기대서관상동맥CGRP、ET-1급NOS적표체변화，차여운동강도관계밀절。인차인위，불동강도운동인기ET—1／CGRP화ET-1／NOS비치적변화가능시운동인기심혈관생물공능개변적인소지일。
Objective： To discuss the effect of different exercise intensity on expressions of calcitonin gene-related peptide （CGRP）, endothelin-1 （ET-1） and nitric oxide synthase （NOS） in the rat coronary artery. Methods： Healthy 3-month male SD rats （n=24） were divided into the control group, small-intensity exercise group and high-intensity exercise group. Exercise group used treadmill exercise to establish the models of small-intensity exercise group and the high-intensity exercise group rats. Immunohistoehemistry of SABC was used to measure difl＇erent exercise intensity of coronary artery CGRP, ET-1, nNOS, iNOS, and eNOS expression of rats. Results： Comparing with the control group, the expressions of CGRP and NOS isofonns in the small-intensity exercise group and the high-intensity exercise group increased significantly, ET-1 expression reduced obviously in the small-intensity exercise group, the ET-1 lev- el of the high-intensity exercise group considerably elevated. The change of the coronary artery CGRP, ET-land NOS expression in the high-intensity exercise group compared with the small-intensity exercise group were significant. The rate of ET-1/CGRP and ET-1/NOS in the groups of small-intensity and high-intensity exereise were lower than the control group. Conclusions： Exercise can induce expression change of CGRP, ET-1 and NOS isoforms in coro- nary artery, and the close relationship with exercise intensity. Therefore the exercise training causes the ET-1/CGRP ratio and ET-1/NOS ratio change is one of the factors that exercise-induced the cardiovascular biological function change.