天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2010年
2期
122~124
,共null页
张洪侠 刘善云 李宁 冯红 王洪涛 王敏 徐新女 王金环
張洪俠 劉善雲 李寧 馮紅 王洪濤 王敏 徐新女 王金環
장홍협 류선운 리저 풍홍 왕홍도 왕민 서신녀 왕금배
耐力运动 ApoE基因敲除小鼠 动脉粥样硬化 一氧化氮 一氧化氮合酶
耐力運動 ApoE基因敲除小鼠 動脈粥樣硬化 一氧化氮 一氧化氮閤酶
내력운동 ApoE기인고제소서 동맥죽양경화 일양화담 일양화담합매
endurance exercise; ApoE-deficient mice; atherosclerosis; nitric oxide; nitric oxide synthase
目的：以ApoE基因敲除（ApoE-/-）小鼠为动脉粥样硬化（AS）模型,观察耐力训练对ApoE-/-小鼠AS形成的干预作用及NO系统的影响,以探讨耐力运动抗AS的可能机制。方法：将24只8周龄ApoE-/-小鼠随机分为AS模型组和运动干预组,每组12只,运动组在活动跑台上进行耐力训练,实验持续14周后,测定两组小鼠主动脉斑块面积及病理变化、血清NO和血脂水平以及主动脉iNOS和eNOS蛋白表达。结果：运动组小鼠主动脉斑块面积显著小于对照组（P〈0.01）,血管壁及内膜损伤程度较对照组减轻,血清NO浓度和主动脉eNOS蛋白表达显著高于对照组（P〈0.01）,i-NOS蛋白表达显著低于对照组（P〈0.05）,血脂水平两组间无显著差异。结论：耐力运动通过增加eNOS蛋白表达、抑制iNOS蛋白表达和提高血中NO水平而发挥抗动脉粥样硬化的作用。
目的：以ApoE基因敲除（ApoE-/-）小鼠為動脈粥樣硬化（AS）模型,觀察耐力訓練對ApoE-/-小鼠AS形成的榦預作用及NO繫統的影響,以探討耐力運動抗AS的可能機製。方法：將24隻8週齡ApoE-/-小鼠隨機分為AS模型組和運動榦預組,每組12隻,運動組在活動跑檯上進行耐力訓練,實驗持續14週後,測定兩組小鼠主動脈斑塊麵積及病理變化、血清NO和血脂水平以及主動脈iNOS和eNOS蛋白錶達。結果：運動組小鼠主動脈斑塊麵積顯著小于對照組（P〈0.01）,血管壁及內膜損傷程度較對照組減輕,血清NO濃度和主動脈eNOS蛋白錶達顯著高于對照組（P〈0.01）,i-NOS蛋白錶達顯著低于對照組（P〈0.05）,血脂水平兩組間無顯著差異。結論：耐力運動通過增加eNOS蛋白錶達、抑製iNOS蛋白錶達和提高血中NO水平而髮揮抗動脈粥樣硬化的作用。
목적：이ApoE기인고제（ApoE-/-）소서위동맥죽양경화（AS）모형,관찰내력훈련대ApoE-/-소서AS형성적간예작용급NO계통적영향,이탐토내력운동항AS적가능궤제。방법：장24지8주령ApoE-/-소서수궤분위AS모형조화운동간예조,매조12지,운동조재활동포태상진행내력훈련,실험지속14주후,측정량조소서주동맥반괴면적급병리변화、혈청NO화혈지수평이급주동맥iNOS화eNOS단백표체。결과：운동조소서주동맥반괴면적현저소우대조조（P〈0.01）,혈관벽급내막손상정도교대조조감경,혈청NO농도화주동맥eNOS단백표체현저고우대조조（P〈0.01）,i-NOS단백표체현저저우대조조（P〈0.05）,혈지수평량조간무현저차이。결론：내력운동통과증가eNOS단백표체、억제iNOS단백표체화제고혈중NO수평이발휘항동맥죽양경화적작용。
Objective： The influence of endurance exercise on the function of preventing from atherosclerosis formation and effects of endurance exercise on NO system in ApoE-deficient mice were investigated to probe possible mechanisms of anti-atherosclerosis by endurance exercise. Methods： Twenty-four 8 week old ApoE-deficient mice were selected and divided into atherosclerosis model group and exercise group randomly. There were twelve mice in each group. Mice of exercise group were trained on treadmill for fourteen weeks. Followings were detected： 1） the square of atherosclerotic plaque and pathologi-cal change; 2） the serum NO and the blood lipid; 3） the protein expression of thoracic aortic iNOS and eNOS. Results： The square of aorta plaque was significantly smaller in ApoE-deficient mice of exercise group than that in mice of control group （P0.01）. The degree of lesion in aorta wall and tunica intima was lighter in ApoE-deficient mice with endurance exercise. The concentration of serum NO and protein expression of aorta eNOS were significantly increased （P0.01） in ApoE-deficient mice with endurance exercise. The protein expression of aorta iNOS was significantly decreased （P0.05） in ApoE-de - ficient mice with endurance exercise. There was no change on the blood lipid. Conclusions： The present study demonstrated that endurance exercise may contribute to its anti-atherosclerosis function by significantly increasing eNOS protein expression and blood NO level as well as inhibiting iNOS protein expression.
