CAJ | 학술논문

目的：通过观察补充海洋肽对骨骼肌细胞骨架蛋白desmin表达的影响，研究补充海洋肽影响运动性骨骼肌损伤修复的机制。方法：72只雄性SD大鼠随机分为安静对照组、运动对照组、运动＋大豆蛋白对照组和运动＋海洋肽实验组，进行6周每周5天的-5°下坡间歇跑训练，速度从20．0m／min增加到35．0m／min，每次10～20min。6周后，大鼠在1次高强度离心运动后即刻、24h和48h处死，取股中肌进行免疫印迹法检测desmin表达量的变化，并取血测血清CK总活性。结果：补充海洋肽和大豆蛋白均可增加desmin蛋白表达量，而且海洋肽增加运动后desmin蛋白表达的量明显优于大豆蛋白。补充海洋肽和大豆蛋白均明显减低CK蛋白从骨骼肌细胞中逸漏出，降低血清CK水平。而且，海洋肽对运动后血清CK的恢复明显早于大豆蛋白。结论：补充海洋肽可维护大鼠高强度离心运动后骨骼肌微结构的完整，促进运动后骨骼肌微结构损伤的修复。
목적：통과관찰보충해양태대골격기세포골가단백desmin표체적영향，연구보충해양태영향운동성골격기손상수복적궤제。방법：72지웅성SD대서수궤분위안정대조조、운동대조조、운동＋대두단백대조조화운동＋해양태실험조，진행6주매주5천적-5°하파간헐포훈련，속도종20．0m／min증가도35．0m／min，매차10～20min。6주후，대서재1차고강도리심운동후즉각、24h화48h처사，취고중기진행면역인적법검측desmin표체량적변화，병취혈측혈청CK총활성。결과：보충해양태화대두단백균가증가desmin단백표체량，이차해양태증가운동후desmin단백표체적량명현우우대두단백。보충해양태화대두단백균명현감저CK단백종골격기세포중일루출，강저혈청CK수평。이차，해양태대운동후혈청CK적회복명현조우대두단백。결론：보충해양태가유호대서고강도리심운동후골격기미결구적완정，촉진운동후골격기미결구손상적수복。
Objective ： The effects of the marine bioactive peptides supplementation on micro - injury of skeletal muscle after intensive training were investigated. Methods ：72 Male Sprague -Dawley rats were randomly divided into non -exercise control group and three training groups： group of supplying marine bioactive peptide, group of supplying soybean protein and group of supplying water. 6 -wk training involves the 5days/wk, and the speed from 20.0m/rain in to 35. 0m/min, and the grade was always -5°. In the seventh week, the tissues were collected from the quadrieeps femoris separately at Oh, 24h and 48h after eccentric exercise. Desmin content determined by Western blotting and Serum entent is higher in group of supplying marine bioactive peptide than in group of supplying soybean protein significantly. So does the concentration of serm enzyme obviously. Conclusions：Marine bioactive peptide supplementation safeguard the structure of myofibre after intensive training, and improved the reconstruction of micro - injury of skeletal muscle.