泉州师范学院学报
泉州師範學院學報
천주사범학원학보
Journal of Quanzhou Normal College
2010年
2期
56~58
,共null页
PCR 日本对虾 β-actin 表达 纯化
PCR 日本對蝦 β-actin 錶達 純化
PCR 일본대하 β-actin 표체 순화
PCR; Penaeus japonicus; β-actin; expression; purification
利用RT-PCR技术从日本对虾中克隆到β-actin基因的cDNA,通过双酶切将其克隆到原核表达载体pGEX-4T-2中,转化大肠杆菌E.coli BL21感受态细胞,获得阳性克隆.4℃下IPTG诱导表达,Glutathione Sepharose 4B纯化后获得高纯度的β-actin蛋白.①
利用RT-PCR技術從日本對蝦中剋隆到β-actin基因的cDNA,通過雙酶切將其剋隆到原覈錶達載體pGEX-4T-2中,轉化大腸桿菌E.coli BL21感受態細胞,穫得暘性剋隆.4℃下IPTG誘導錶達,Glutathione Sepharose 4B純化後穫得高純度的β-actin蛋白.①
이용RT-PCR기술종일본대하중극륭도β-actin기인적cDNA,통과쌍매절장기극륭도원핵표체재체pGEX-4T-2중,전화대장간균E.coli BL21감수태세포,획득양성극륭.4℃하IPTG유도표체,Glutathione Sepharose 4B순화후획득고순도적β-actin단백.①
β-actin of Penaeus japonicus was cloned by RT-PCR and cloned into pGEX-4T-2 plasmid.This plasmid was used to transfer E.coli BL21.After induced with IPTG at 4 ℃,the fusion protein was purified with Glutathione Sepharose 4B.
