泉州师范学院学报
泉州師範學院學報
천주사범학원학보
Journal of Quanzhou Normal College
2010年
2期
59~62
,共null页
李夏 袁建军 卢英华 熊裕焱 姚传义 敬科举
李夏 袁建軍 盧英華 熊裕焱 姚傳義 敬科舉
리하 원건군 로영화 웅유염 요전의 경과거
苹果酸酶基因 简并PCR Code Hop 巢式PCR
蘋果痠酶基因 簡併PCR Code Hop 巢式PCR
평과산매기인 간병PCR Code Hop 소식PCR
malic enzyme gene; degenerate PCR; CodeHop; nested PCR
根据已报道的酵母苹果酸酶基因序列,利用CodeHop（Consensus Degenerate Hybrid Oligonucleotide Primers）软件设计两对简并引物,以斯达油脂酵母（L.starkeyi CICC 1809）总DNA为模板做简并PCR,得到2个基因片段（ME1,ME2）.对这2个目的片段进行测序,将结果翻译成氨基酸序列,blastp结果显示其中ME2片段为油脂酵母未报道苹果酸酶基因的序列（Gene Bank登录号GU348991）,并对巢式PCR方法检验简并PCR结果的有效性进行了评估.
根據已報道的酵母蘋果痠酶基因序列,利用CodeHop（Consensus Degenerate Hybrid Oligonucleotide Primers）軟件設計兩對簡併引物,以斯達油脂酵母（L.starkeyi CICC 1809）總DNA為模闆做簡併PCR,得到2箇基因片段（ME1,ME2）.對這2箇目的片段進行測序,將結果翻譯成氨基痠序列,blastp結果顯示其中ME2片段為油脂酵母未報道蘋果痠酶基因的序列（Gene Bank登錄號GU348991）,併對巢式PCR方法檢驗簡併PCR結果的有效性進行瞭評估.
근거이보도적효모평과산매기인서렬,이용CodeHop（Consensus Degenerate Hybrid Oligonucleotide Primers）연건설계량대간병인물,이사체유지효모（L.starkeyi CICC 1809）총DNA위모판주간병PCR,득도2개기인편단（ME1,ME2）.대저2개목적편단진행측서,장결과번역성안기산서렬,blastp결과현시기중ME2편단위유지효모미보도평과산매기인적서렬（Gene Bank등록호GU348991）,병대소식PCR방법검험간병PCR결과적유효성진행료평고.
According to the known sequences of malic enzyme gene of yeast,two pairs of primers were designed by CodeHop（Consensus Degenerate Hybrid Oligonucleotide Primers）.Two specific DNA fragments were amplified with polymerase chain reaction（PCR） assay from genome DNA of L.starkeyi CICC 1809.Those DNA fragments（ME1,ME2） were sequenced and translated into corresponding amino acid sequences.The ME2 sequence of the two fragment was determined as the unknown malic enzyme gene fragment by alignment to the reported malic enzyme sequences（Genebank number is GU348991）.This study also verified the accuracy and effectiveness of the results with the nested-polymerase chain reaction（PCR）.
