体育科学
體育科學
체육과학
China Sport Science
2010年
7期
56~61
,共null页
李海鹏 王立丰 关尚一 马景亮 丁树哲 卢健
李海鵬 王立豐 關尚一 馬景亮 丁樹哲 盧健
리해붕 왕립봉 관상일 마경량 정수철 로건
肌肉衰减征 爬梯运动 线粒体 细胞凋亡 鼠 动物实验
肌肉衰減徵 爬梯運動 線粒體 細胞凋亡 鼠 動物實驗
기육쇠감정 파제운동 선립체 세포조망 서 동물실험
Sarcopenia ; ladder climbing ; mitochondria ; apoptosis ; rat ; animal experiment
目的:探讨Sareopenia关联的线粒体介导的细胞凋亡信号通路中各凋亡基因的增龄变化情况及爬梯运动对其的影响。方法:以快速老化(Senescence-accelerated mice prone/8,SAMP8)小鼠为衰老动物模型,将其分为青年安静组(YC组)、老年安静组(OC组)和老年爬梯运动组(OR纽),采用实时荧光定量PCR的方法,分别对各组腓肠肌Caspase依赖性凋亡通路中相关基因(CytC、apaf-1、Caspase-9和Caspase-3)和Caspase非依赖性凋亡通路中相关基因(脚、AIF和EndoG)进行检测。结果:0C组腓肠肌Sarcopenia Index(SI)值显著低于YC组;随增龄OC纽腓肠肌中apaf-1、Caspase-3和PARP的表达均显著上调,而A咿却显著下调;爬梯运动能够下调OR组腓肠肌中CytC和Caspase-3的表达,而A匝和EndoG却未见显著性变化。结论:老年SAMP8小鼠腓肠肌可作为Sarcopema研究模型;Sarcopenia关联的线粒体介导的细胞凋亡信号通路中Caspase依赖与Caspase非依赖两条凋亡途径发挥的作用存在一定差异,衰老骨骼肌质量衰减与否以及衰减程度最终有赖于两条途径之间的对抗;8周爬梯运动能够一定程度上弱化老年小鼠腓肠肌中的凋亡信号,避免其更大范围地进入凋亡程序而加剧Sarcopenia,对“脆弱”的骨骼肌起到一定的保护作用。
目的:探討Sareopenia關聯的線粒體介導的細胞凋亡信號通路中各凋亡基因的增齡變化情況及爬梯運動對其的影響。方法:以快速老化(Senescence-accelerated mice prone/8,SAMP8)小鼠為衰老動物模型,將其分為青年安靜組(YC組)、老年安靜組(OC組)和老年爬梯運動組(OR紐),採用實時熒光定量PCR的方法,分彆對各組腓腸肌Caspase依賴性凋亡通路中相關基因(CytC、apaf-1、Caspase-9和Caspase-3)和Caspase非依賴性凋亡通路中相關基因(腳、AIF和EndoG)進行檢測。結果:0C組腓腸肌Sarcopenia Index(SI)值顯著低于YC組;隨增齡OC紐腓腸肌中apaf-1、Caspase-3和PARP的錶達均顯著上調,而A咿卻顯著下調;爬梯運動能夠下調OR組腓腸肌中CytC和Caspase-3的錶達,而A匝和EndoG卻未見顯著性變化。結論:老年SAMP8小鼠腓腸肌可作為Sarcopema研究模型;Sarcopenia關聯的線粒體介導的細胞凋亡信號通路中Caspase依賴與Caspase非依賴兩條凋亡途徑髮揮的作用存在一定差異,衰老骨骼肌質量衰減與否以及衰減程度最終有賴于兩條途徑之間的對抗;8週爬梯運動能夠一定程度上弱化老年小鼠腓腸肌中的凋亡信號,避免其更大範圍地進入凋亡程序而加劇Sarcopenia,對“脆弱”的骨骼肌起到一定的保護作用。
목적:탐토Sareopenia관련적선립체개도적세포조망신호통로중각조망기인적증령변화정황급파제운동대기적영향。방법:이쾌속노화(Senescence-accelerated mice prone/8,SAMP8)소서위쇠로동물모형,장기분위청년안정조(YC조)、노년안정조(OC조)화노년파제운동조(OR뉴),채용실시형광정량PCR적방법,분별대각조비장기Caspase의뢰성조망통로중상관기인(CytC、apaf-1、Caspase-9화Caspase-3)화Caspase비의뢰성조망통로중상관기인(각、AIF화EndoG)진행검측。결과:0C조비장기Sarcopenia Index(SI)치현저저우YC조;수증령OC뉴비장기중apaf-1、Caspase-3화PARP적표체균현저상조,이A이각현저하조;파제운동능구하조OR조비장기중CytC화Caspase-3적표체,이A잡화EndoG각미견현저성변화。결론:노년SAMP8소서비장기가작위Sarcopema연구모형;Sarcopenia관련적선립체개도적세포조망신호통로중Caspase의뢰여Caspase비의뢰량조조망도경발휘적작용존재일정차이,쇠로골격기질량쇠감여부이급쇠감정도최종유뢰우량조도경지간적대항;8주파제운동능구일정정도상약화노년소서비장기중적조망신호,피면기경대범위지진입조망정서이가극Sarcopenia,대“취약”적골격기기도일정적보호작용。
Objective : To discuss the change of apoptotic genes in Sarcopenia related mitoehondria mediated apoptotic signaling pathway, and the effects of ladder climbing exercise on it. Methods:SAMP8 mice are selected as the aging model animal, with the grouping of young control group (YC),old control group (OC) and old resistant exercise group (OR). The method of real-time PCR is used to detect the changes of apoptotic genes, such as Cyt C, apaf-1, Caspase- 9, Caspase-3, PARP, AIF and Endo G in each group. Results: (1) Value of SI in gastroenemius of OC group is lower than that of YC group significantly; (2) Expression of apaf-1, Caspase-3 and PARP increase significantly with aging, on the contrary the expression of AIF decreases significantly; (3) Ladder climbing exercise down-regulates the expression of Cyt C and Caspase-3, but has no effect on AIF and Endo G. Conclusion: (1) The gastrocnernius of senile SAMP8 mice can be used as a model for the research of Sarcopenia; (2) Sarcopenia related mitochondrial mediated Caspase dependent and independent apoptotic signaling pathways show different directions during apoptotic selection, therefore, the final result, Sarcopenia or not, depends on the antagonism between the two pathways; (3) Eight weeks ladder climbing can attenuate the inclination of the apoptosis in the frail senile mice to avoid further advancing for apoptosis and Sarcopenia finally.