天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2010年
4期
340~343
,共null页
大鼠 过度训练 L-精氨酸 肌糖原 AMP-活化的蛋白激酶 葡萄糖载体蛋白-4
大鼠 過度訓練 L-精氨痠 肌糖原 AMP-活化的蛋白激酶 葡萄糖載體蛋白-4
대서 과도훈련 L-정안산 기당원 AMP-활화적단백격매 포도당재체단백-4
rat; overtraining; L-arginine; muscle glycogen; GLUT4; AMPK
目的：观察体外补充L-精氨酸对过度训练大鼠的作用。方法：9周龄SD雌性大鼠40只,随机分为安静对照组（A组）、大强度运动组（B组）、大强度运动服L-精氨酸组（C组）、过度训练组（D组）,其中D1组为过度训练组,D2组为过度训练加服L-精氨酸组。上坡跑训练9周后测各组白腓肠肌和比目鱼肌的肌糖原含量、AMPK活性和肌膜GLUT-4含量。结果：B组和D1组糖原含量较A组有升高趋势,但无显著性;C组显著高于A组（P〈0.01）;D2组大鼠比目鱼肌糖原含量较A组显著升高（P〈0.01）;D2组较D1组升高,但未达到显著水平（P〉0.05）。B组、C组和D2组AMPK活性显著高于A组（P〈0.01）;D2组较D1组有明显增加,差异显著（P〈0.05）。B组、C组和D2组GLUT-4含量分别较A组增加100%左右,D1组增加11%;D2组较D1组增加57%。结论：体外补充L-精氨酸可能有利于延缓或治疗过度训练。
目的：觀察體外補充L-精氨痠對過度訓練大鼠的作用。方法：9週齡SD雌性大鼠40隻,隨機分為安靜對照組（A組）、大彊度運動組（B組）、大彊度運動服L-精氨痠組（C組）、過度訓練組（D組）,其中D1組為過度訓練組,D2組為過度訓練加服L-精氨痠組。上坡跑訓練9週後測各組白腓腸肌和比目魚肌的肌糖原含量、AMPK活性和肌膜GLUT-4含量。結果：B組和D1組糖原含量較A組有升高趨勢,但無顯著性;C組顯著高于A組（P〈0.01）;D2組大鼠比目魚肌糖原含量較A組顯著升高（P〈0.01）;D2組較D1組升高,但未達到顯著水平（P〉0.05）。B組、C組和D2組AMPK活性顯著高于A組（P〈0.01）;D2組較D1組有明顯增加,差異顯著（P〈0.05）。B組、C組和D2組GLUT-4含量分彆較A組增加100%左右,D1組增加11%;D2組較D1組增加57%。結論：體外補充L-精氨痠可能有利于延緩或治療過度訓練。
목적：관찰체외보충L-정안산대과도훈련대서적작용。방법：9주령SD자성대서40지,수궤분위안정대조조（A조）、대강도운동조（B조）、대강도운동복L-정안산조（C조）、과도훈련조（D조）,기중D1조위과도훈련조,D2조위과도훈련가복L-정안산조。상파포훈련9주후측각조백비장기화비목어기적기당원함량、AMPK활성화기막GLUT-4함량。결과：B조화D1조당원함량교A조유승고추세,단무현저성;C조현저고우A조（P〈0.01）;D2조대서비목어기당원함량교A조현저승고（P〈0.01）;D2조교D1조승고,단미체도현저수평（P〉0.05）。B조、C조화D2조AMPK활성현저고우A조（P〈0.01）;D2조교D1조유명현증가,차이현저（P〈0.05）。B조、C조화D2조GLUT-4함량분별교A조증가100%좌우,D1조증가11%;D2조교D1조증가57%。결론：체외보충L-정안산가능유리우연완혹치료과도훈련。
Objective： The purpose of the present study was to observe effect of supplying L-arginine on skeletal muscle glycogen contents,AMP-activated protein kinase（AMPK） activity and sarcolemma Glucose transporter type 4（GLUT4） protein contents in overtraining rats.Methods： Forty SD rats were randomly divided into group A（sedentary）,group B（underwent endurance exercise 60 minutes/day）,group C（underwent endurance exercise 60 minutes/day,supplying L-arginine） and group D（underwent endurance exercise 120 minutes/day）,group D subdivided group D1（without L-arginine） and group D2（with L-arginine）.All exercise groups were trained 6 days/wk for 9 weeks.Muscle glycogen,sarcolemma GLUT4 contents and muscle AMPK activity were determined after the 9-week training.Results： Muscle glycogen contents in group B and group D1 exist a trend of increase compared with that in group A,but not significantly（P0.05）.Muscle glycogen contents in soleus showed a significant higher level in group C and group D2 compared with that in group A（P0.01）,and it showed a trend of increase in group D2 compared with that in group D1,but not significantly（P0.05）.Muscle AMPK activity in group B,group C and group D2 increased significantly compared with that in group A,and it showed a significant higher level in group D2 compared with that in group D1（P0.01）.Sarcolemma GLUT4 contents in group B,group C and group D2 increased about 100% compared with that in group A,but it only increased 11% in group D1.And that increased by 57% in group D2 as compared with that in group D1.Conclusion： Supplying with L-Arg could increase muscle glycogen cotents,muscle AMPK activity,and sarcolemma GLUT4 content in overtraining rats,which might be helpful in staying or treating overtraining.
