天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2010年
5期
388~391
,共null页
负重训练 大豆多肽 骨骼肌 α-肌动蛋白 肌球蛋白重链异形体
負重訓練 大豆多肽 骨骼肌 α-肌動蛋白 肌毬蛋白重鏈異形體
부중훈련 대두다태 골격기 α-기동단백 기구단백중련이형체
weight training; soy polypeptide; skeletal muscle; α-actin; myosin heavy chain isoforms
目的：探讨负重训练和补充大豆多肽对D-半乳糖衰老大鼠骨骼肌α-actin mRNA和MHC异形体mRNA表达的影响。方法：56只3月龄雄性SD大鼠随机分为7组、每组8只、成年组、模型组、小负重组、大负重组、补肽组、补肽小负重组、补肽大负重组。除成年组外,其余各组进行6周D-半乳糖造模同时施加相应大、小负重的跑台训练和补肽干预,6周后处死大鼠,测试各项指标。结果：与成年组相比,模型组大鼠骨骼肌α-actin、MHC-I、MHC-IIa、MHC-IIxmRNA表达量显著下降（P〈0.01）,MHC-IIbmRNA的表达量显著升高（P〈0.01）,负重训练或补肽干预均可以有效缓解这种骨骼肌衰老性的表现,两种方法具有显著的交互作用（P〈0.01或P〈0.05）。结论：负重训练或补充大豆多肽均可以有效缓解衰老大鼠骨骼肌α-actin和MHC异形体mRNA表达量的衰老性表现,并且两种方法联合应用效果好于单一干预因素。
目的：探討負重訓練和補充大豆多肽對D-半乳糖衰老大鼠骨骼肌α-actin mRNA和MHC異形體mRNA錶達的影響。方法：56隻3月齡雄性SD大鼠隨機分為7組、每組8隻、成年組、模型組、小負重組、大負重組、補肽組、補肽小負重組、補肽大負重組。除成年組外,其餘各組進行6週D-半乳糖造模同時施加相應大、小負重的跑檯訓練和補肽榦預,6週後處死大鼠,測試各項指標。結果：與成年組相比,模型組大鼠骨骼肌α-actin、MHC-I、MHC-IIa、MHC-IIxmRNA錶達量顯著下降（P〈0.01）,MHC-IIbmRNA的錶達量顯著升高（P〈0.01）,負重訓練或補肽榦預均可以有效緩解這種骨骼肌衰老性的錶現,兩種方法具有顯著的交互作用（P〈0.01或P〈0.05）。結論：負重訓練或補充大豆多肽均可以有效緩解衰老大鼠骨骼肌α-actin和MHC異形體mRNA錶達量的衰老性錶現,併且兩種方法聯閤應用效果好于單一榦預因素。
목적：탐토부중훈련화보충대두다태대D-반유당쇠로대서골격기α-actin mRNA화MHC이형체mRNA표체적영향。방법：56지3월령웅성SD대서수궤분위7조、매조8지、성년조、모형조、소부중조、대부중조、보태조、보태소부중조、보태대부중조。제성년조외,기여각조진행6주D-반유당조모동시시가상응대、소부중적포태훈련화보태간예,6주후처사대서,측시각항지표。결과：여성년조상비,모형조대서골격기α-actin、MHC-I、MHC-IIa、MHC-IIxmRNA표체량현저하강（P〈0.01）,MHC-IIbmRNA적표체량현저승고（P〈0.01）,부중훈련혹보태간예균가이유효완해저충골격기쇠로성적표현,량충방법구유현저적교호작용（P〈0.01혹P〈0.05）。결론：부중훈련혹보충대두다태균가이유효완해쇠로대서골격기α-actin화MHC이형체mRNA표체량적쇠로성표현,병차량충방법연합응용효과호우단일간예인소。
Objective：To study of the effect of weight training and soy polypeptide supplement on α-actin mRNA and MHC isoforms mRNA of D-galactose of aging rat skeletal muscle.Method：56 3-month male SD rats were randomly divided into 7 groups,8 in each group：adult group,model group,small load exercise group,big load exercise group,peptide group,peptide and small load exercise group,peptide and big load exercise group.All the groups were modeled in 6-week D-galactose injection and interfered in the corresponding big and small weight run training and soy polypeptide supplement ex-cept adult group.The rats were killed after 6 weeks,and tested the indicators.Result：Compared with the adult group,the α-actin,MHC-I,MHC-IIa,MHC-IIx mRNA expression in rats skeletal muscle of model group were significantly decreased（P〈0.01）,MHC-IIb mRNA expression was significantly increased（P〈0.01）,weight training or soy polypeptide supplement could effectively alleviate the aging performance of skeletal muscle,they had notable interaction（P〈0.01 or P〈0.05）.Conclusions：Weight training or soy polypeptide supplement may alleviate the age-related performance of aging rats skele-tal muscle in α-actin and MHC isoform mRNA expression effectively,the combined effect of two methods was better than a single intervention factor.
