上海体育学院学报
上海體育學院學報
상해체육학원학보
Journal of Shanghai Physical Education Institute
2010年
6期
43~45
,共null页
机械生长因子 信使核糖核酸 荧光定量RT—PCR 检测方法
機械生長因子 信使覈糖覈痠 熒光定量RT—PCR 檢測方法
궤계생장인자 신사핵당핵산 형광정량RT—PCR 검측방법
mechano growth factor; mRNA; real-timequantitative RT-PCR; detection method
目的：建立机械生长因子（MGF）基因表达的实时荧光定量RT—PCR方法。方法：应用实时定量RT-PCR法检测腓肠肌中MGF基因表达。结果及结论：荧光定量RT—PCR法能绝对定量MGF兴奋荆基因表达水平，相关系数大于0．99。
目的：建立機械生長因子（MGF）基因錶達的實時熒光定量RT—PCR方法。方法：應用實時定量RT-PCR法檢測腓腸肌中MGF基因錶達。結果及結論：熒光定量RT—PCR法能絕對定量MGF興奮荊基因錶達水平，相關繫數大于0．99。
목적：건립궤계생장인자（MGF）기인표체적실시형광정량RT—PCR방법。방법：응용실시정량RT-PCR법검측비장기중MGF기인표체。결과급결론：형광정량RT—PCR법능절대정량MGF흥강형기인표체수평，상관계수대우0．99。
Objective： Establish the real-time quantitative RT- PCR method for detecting the gene expression of mechano growth factor （MGF）. Methods： The MGF gene expression in gastrocnemius was detected by the real-time quantitative RT-PCR method. Results： The MGF mRNA expression in gastrocnemins could be absolutely measured by the real-time quantitative RT- PCR method with the correlation coefficient （ r 〉 0.99 ）.
