韶关学院学报
韶關學院學報
소관학원학보
Journal of Shaoguan University(Social Science Edition)
2010年
9期
50~55
,共null页
毛细管电泳（CE） 杀虫剂 氧化乐果 含量检测
毛細管電泳（CE） 殺蟲劑 氧化樂果 含量檢測
모세관전영（CE） 살충제 양화악과 함량검측
capillary electrophoresis; omethoate; content determination
应用高效毛细管电泳法对氧化乐果的含量进行了测定,研究了检测波长、缓冲体系、缓冲液pH、缓冲液浓度、SDS浓度和分离电压对氧化乐果测定的影响.结果表明,在pH为7.5、20 mmol/L NaH2PO4-Na2HPO4缓冲液、10 mmol/L SDS、209 nm、25 kV的条件下,氧化乐果的测定效果最佳.测定氧化乐果的检测限为0.15μg/mL,线性范围为0.5～30μg/mL.采用标准加入法,测定回收率为92%～105%.该方法可成功应用于农田水样中氧化乐果含量的测定.
應用高效毛細管電泳法對氧化樂果的含量進行瞭測定,研究瞭檢測波長、緩遲體繫、緩遲液pH、緩遲液濃度、SDS濃度和分離電壓對氧化樂果測定的影響.結果錶明,在pH為7.5、20 mmol/L NaH2PO4-Na2HPO4緩遲液、10 mmol/L SDS、209 nm、25 kV的條件下,氧化樂果的測定效果最佳.測定氧化樂果的檢測限為0.15μg/mL,線性範圍為0.5～30μg/mL.採用標準加入法,測定迴收率為92%～105%.該方法可成功應用于農田水樣中氧化樂果含量的測定.
응용고효모세관전영법대양화악과적함량진행료측정,연구료검측파장、완충체계、완충액pH、완충액농도、SDS농도화분리전압대양화악과측정적영향.결과표명,재pH위7.5、20 mmol/L NaH2PO4-Na2HPO4완충액、10 mmol/L SDS、209 nm、25 kV적조건하,양화악과적측정효과최가.측정양화악과적검측한위0.15μg/mL,선성범위위0.5～30μg/mL.채용표준가입법,측정회수솔위92%～105%.해방법가성공응용우농전수양중양화악과함량적측정.
A method for the determination of omethoate by capillary electrophoresis has been developed in this paper.The conditions for omethoate determination,such as inspection wavelength,buffer system,buffer pH value,buffer concentration,SDS concentration and applied voltage were investigated.The optimum conditions for omethoate determination were inspect wavelength of 209 nm,phosphate buffer,buffer pH of 7.5,buffer concentration of 20 mmol/L,SDS concentration of 10 mmol/L,applied voltage of 25 kV.Under the optimum conditions,detection limits was 0.15 μg/mL,linearity was 0.5~30 μg/mL,recovery was between 92%~105% for standard addition.The method was applied to the detection of omethoate in water successfully.
