CAJ | 학술논문

以SD雄性大鼠为材料,采用慢性轻度不可预见性应激＋孤养方法复制大鼠抑郁模型,比较促肾上腺皮质激素释放激素（CRH）免疫阳性神经元在大鼠大脑皮质的区域分布特征。结果显示：CRH免疫阳性神经元在皮层的各层均有分布;与对照组（NC组）相比,模型组（MC组）大鼠CRH免疫阳性细胞在大脑皮质的运动皮质（M）、感觉皮质（S）、听皮质（Au）、梨形皮质（Pir）显著增加,嗅皮质（Ol）也有增加但不明显。结论：CRH免疫反应阳性细胞在大脑皮层内广泛分布,抑郁大鼠CRH在皮质的表达增加,提示其可能参与应激反应。
이SD웅성대서위재료,채용만성경도불가예견성응격＋고양방법복제대서억욱모형,비교촉신상선피질격소석방격소（CRH）면역양성신경원재대서대뇌피질적구역분포특정。결과현시：CRH면역양성신경원재피층적각층균유분포;여대조조（NC조）상비,모형조（MC조）대서CRH면역양성세포재대뇌피질적운동피질（M）、감각피질（S）、은피질（Au）、리형피질（Pir）현저증가,후피질（Ol）야유증가단불명현。결론：CRH면역반응양성세포재대뇌피층내엄범분포,억욱대서CRH재피질적표체증가,제시기가능삼여응격반응。
To explore distribution characteristics of immunopositive neurons for the corticotrophin releasing hormone（CRH）in the cerebral cortex of rats as well as changes in depressed rats.Methods：20 SD male rats were randomized into control group（NC group）,model control group（MC group）,with 10rats in each group.The depression model was achieved by chronic unpredictable mild stress plus solitary breeding.The immunohistochemistry method was used to observe immunopositive neurons for CRH in different regions of the cerebral cortex of the rats,comparing distribution differences of the neurons in cortex regions of rats in both groups.Results：Immunopositive neurons for CRH were distributed in all layers of the cortex.Compared to rats in the NC group,rats in the MC group significantly increased their expression of CRH in the cortex,particularly in the motor cortex（M）,sensory cortex（S）,auditory cortex（Au）,and pyriform cortex（Pir）.The CRH expression in the olfactory cortex （Ol）was also intensified but not significant.Conclusions：Immunopositive neurons for CRH were extensively distributed in the cerebral cortex of the rats and the depressed rats increased the expression of CRH in the cortex,suggesting their involvement with stress response.