北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2011年
2期
52~54
,共null页
潘同斌 钱福鸿 王晓雪 王雅一
潘同斌 錢福鴻 王曉雪 王雅一
반동빈 전복홍 왕효설 왕아일
股骼肌 基因表达 肌球蛋白重链 成肌调节因子
股骼肌 基因錶達 肌毬蛋白重鏈 成肌調節因子
고격기 기인표체 기구단백중련 성기조절인자
gene expression; comparison; myosin heavy chain（MHC） isoforms; myogenic regulation factors（MRFs）
目的：比较测定几种不同动物骨骼肌肌球蛋白重链（MHC）亚型及成肌调节因子（MRFs）基因表达,引物均采用大鼠基因号设计,旨在比较观察其它动物与大鼠基因表达的交叉及相似度。方法：选用大鼠、家兔、牛与人的股外侧肌样本各6个,迅速用TRIZOL试剂匀浆固定,采用实时荧光-PCR法,测定骨骼肌MHCI,IIa,IIx,IIb四种亚型及成肌调节因子（MRFs）的mRNA基因表达。结果：未经内参较正的测定值,人、牛、兔的肌球蛋白重链（MHC）亚型及成肌调节因子（MRFs）基因表达均明显低于大鼠（P〈0.01）,提示各物种的同种基因存在差异性,其引物特异性较强;但经过内参校正后的测定值,人的MHC各亚型基因表达与大鼠含量较接近,有一定的交叉和基因相似性;而牛的含量虽可检出,但仍明显低于大鼠含量（P〈0.01）,表明其基因相似性较低;兔与大鼠基因未表现出相似性。提示,各物种同种基因的表达既存在差异性,又有一定的交叉和相似性。其蛋白水平的比较测定有待进一步研究。
目的：比較測定幾種不同動物骨骼肌肌毬蛋白重鏈（MHC）亞型及成肌調節因子（MRFs）基因錶達,引物均採用大鼠基因號設計,旨在比較觀察其它動物與大鼠基因錶達的交扠及相似度。方法：選用大鼠、傢兔、牛與人的股外側肌樣本各6箇,迅速用TRIZOL試劑勻漿固定,採用實時熒光-PCR法,測定骨骼肌MHCI,IIa,IIx,IIb四種亞型及成肌調節因子（MRFs）的mRNA基因錶達。結果：未經內參較正的測定值,人、牛、兔的肌毬蛋白重鏈（MHC）亞型及成肌調節因子（MRFs）基因錶達均明顯低于大鼠（P〈0.01）,提示各物種的同種基因存在差異性,其引物特異性較彊;但經過內參校正後的測定值,人的MHC各亞型基因錶達與大鼠含量較接近,有一定的交扠和基因相似性;而牛的含量雖可檢齣,但仍明顯低于大鼠含量（P〈0.01）,錶明其基因相似性較低;兔與大鼠基因未錶現齣相似性。提示,各物種同種基因的錶達既存在差異性,又有一定的交扠和相似性。其蛋白水平的比較測定有待進一步研究。
목적：비교측정궤충불동동물골격기기구단백중련（MHC）아형급성기조절인자（MRFs）기인표체,인물균채용대서기인호설계,지재비교관찰기타동물여대서기인표체적교차급상사도。방법：선용대서、가토、우여인적고외측기양본각6개,신속용TRIZOL시제균장고정,채용실시형광-PCR법,측정골격기MHCI,IIa,IIx,IIb사충아형급성기조절인자（MRFs）적mRNA기인표체。결과：미경내삼교정적측정치,인、우、토적기구단백중련（MHC）아형급성기조절인자（MRFs）기인표체균명현저우대서（P〈0.01）,제시각물충적동충기인존재차이성,기인물특이성교강;단경과내삼교정후적측정치,인적MHC각아형기인표체여대서함량교접근,유일정적교차화기인상사성;이우적함량수가검출,단잉명현저우대서함량（P〈0.01）,표명기기인상사성교저;토여대서기인미표현출상사성。제시,각물충동충기인적표체기존재차이성,우유일정적교차화상사성。기단백수평적비교측정유대진일보연구。
Objective： To compare the similarity of the gene expression of myosin heavy chain（MHC） isoforms and myogenic regulation factors（MRFs） in skeletal muscle among different animals by the primer of rat.Method： 24 samples of vastus lateralis respectively from rats,rabbits,bulls and human were fixed and homogenated with TRIZOL.Then the gene expression of MHC isoforms and MRFs in skeletal muscle among different animals was measured by realtime PCR method.Results：When not being calibrated by internal reference,the gene expressions of MHC isoforms and MRFs in skeletal muscles of rabbits,bulls and human were significantly lower than ones in rats（P0.01）.This indicated that there are primer specialties among different animals.But when being calibrated by internal reference,the mRNA values of MHC isoforms in human were close to the ones in rat with the high similarity of gene.At same time,the mRNA values of MHC isoforms in bull were significantly lower than in rat（P0.01） with a low similarity of gene.On the other hand,there were no similarity of the genes between rabbit and rat. Indications： the expressions of the same gene of different species have differences,while they also share overlapping features and similarity.The comparison of the protein expression must be researched in next experiment.
