CAJ | 학술논문

为探讨一次大强度耐力运动过程中,AMPK活性变化对骨骼肌蛋白质降解的作用。将36只SD大鼠进行一次跑台运动,坡度5%,运动强度25 m/min,运动时间60 min。取样为运动前、运动0.5、1 h,运动后1、2、6 h等6个点。使用高效液相色谱法测定AMP、ATP质量摩尔浓度;采用同位素技术测定腓肠肌中AMPK活性的变化;采用荧光定量PCR技术,测定腓肠肌中MuRF1、MAFbx基因表达量的变化。结果发现：（1）运动0.5 h到运动后即刻,AMP质量摩尔浓度及AMP与ATP质量摩尔浓度比值升高（P〈0.05）,运动后1、2、6 h组,腓肠肌AMP质量摩尔浓度及AMP、ATP质量摩尔浓度比值与安静组比较差异没有显著性;ATP质量摩尔浓度各组变化不大,差异没有显著性。（2）AMPK活性在运动0.5 h后开始升高,运动后2 h达到最高,运动后6 h开始下降但还高于对照组。（3）与安静组比较,运动0.5 h组、运动1 h组MuRF1 mRNA、MAFbx mRNA表达量差异没有显著性;运动后1 h、2 h组MuRF1 mRNA、MAFbx mRNA表达量与安静组比较升高,差异有非常显著性（P〈0.01）,分别升高1.98、3.57和1.95、2.55倍;运动后6 h组MuRF1 mRNA、MAFbx mRNA表达量与安静组比较升高,差异有显著性（P〈0.05）。结果说明：一次性大强度耐力运动后1~6 h,骨骼肌蛋白质降解可能增强,其原因可能是AMPK活化,促进MAFbx mRNA、MuRF1 mRNA基因表达,促进骨骼肌蛋白质的降解。
위탐토일차대강도내력운동과정중,AMPK활성변화대골격기단백질강해적작용。장36지SD대서진행일차포태운동,파도5%,운동강도25 m/min,운동시간60 min。취양위운동전、운동0.5、1 h,운동후1、2、6 h등6개점。사용고효액상색보법측정AMP、ATP질량마이농도;채용동위소기술측정비장기중AMPK활성적변화;채용형광정량PCR기술,측정비장기중MuRF1、MAFbx기인표체량적변화。결과발현：（1）운동0.5 h도운동후즉각,AMP질량마이농도급AMP여ATP질량마이농도비치승고（P〈0.05）,운동후1、2、6 h조,비장기AMP질량마이농도급AMP、ATP질량마이농도비치여안정조비교차이몰유현저성;ATP질량마이농도각조변화불대,차이몰유현저성。（2）AMPK활성재운동0.5 h후개시승고,운동후2 h체도최고,운동후6 h개시하강단환고우대조조。（3）여안정조비교,운동0.5 h조、운동1 h조MuRF1 mRNA、MAFbx mRNA표체량차이몰유현저성;운동후1 h、2 h조MuRF1 mRNA、MAFbx mRNA표체량여안정조비교승고,차이유비상현저성（P〈0.01）,분별승고1.98、3.57화1.95、2.55배;운동후6 h조MuRF1 mRNA、MAFbx mRNA표체량여안정조비교승고,차이유현저성（P〈0.05）。결과설명：일차성대강도내력운동후1~6 h,골격기단백질강해가능증강,기원인가능시AMPK활화,촉진MAFbx mRNA、MuRF1 mRNA기인표체,촉진골격기단백질적강해。
The authors probed into functions played by changes of AMPK activity in the degradation of skeleton muscle protein during one time high intensity endurance exercising.Method： let 36 SD rats do a one time treadmill exercise at a slope of 5%,an exercise intensity of 25 m/min,and an exercise time of 60min.Sampling times were before exercising,after 0.5h and 1h of exercising,1 h,2 h and 6 h after exercising.The authors measured AMP and ATP molalities by using high efficiency liquid chromatography,changes of AMPK activity in gastrocnemius muscle by using isotope technology,and changes of MuRF1 and MAFbx gene expressions in gastrocnemius muscle by using fluorescence quantification PCR technology.Results： 1） from the moment after 0.5 h of exercising to the movement immediately after exercising,AMP molality and the ratio of AMP molality to ATP molality increased （P0.05）;there was no significant difference in AMP molality and the ratio of AMP molality to ATP molality in gastrocnemius muscle between 1 h,2 h and 6h after exercising groups and the calm group;there was no significant change or difference in ATP molality between various groups;2） AMPK activity started to increase after 1.5 h of exercising,reached its peak at 2 h after exercising,started to lower at 6 h after exercising but was still higher than that of rats in the control group;3） there was no significant difference in MuRF1 mRNA and MAFbx mRNA expression volumes between after 0.5h and 1h of exercising groups and the calm group;compared with those of rats in the calm group,MuRF1 mRNA and MAFbx mRNA expression volumes of rats in 1h and 2 h after exercising groups increased by 1.98 times,3.57 times and 1.95 times 2.55 times respectively,and the differences were very significant（P0.01）;compared with those of rats in the calm group,MuRF1 mRNA and MAFbxmRNA expression volumes of rats in the 6h after exercising group increased,and the differences were significant（P0.05）.The results indicate that in 1-6 h after one time high intensity endurance exercising,the degradation of skeletal muscle protein may be intensified,possibly by MAFbx mRNA and MuRF1 mRNA gene expressions promoted by AMPK activation.