CAJ | 학술논문

目的：报道了补充虫源壳聚糖对大鼠肝脏抗氧化酶系和SOD基因表达影响的研究结果。方法：对大负荷游泳训练大鼠补充剂量分别为各组平均体重的0.5 g/kg、1.0 g/kg和2.0 g/kg的虫源壳聚糖,在8周大负荷训练结束后测定肝脏抗氧化酶系的活性,并分析不同处理组动物肝脏SOD基因表达水平差异。结果：补充虫源壳聚糖能够显著提高抗氧化酶系活性,虫源壳聚糖0.5 g/kg补充组与单纯大负荷运动组相比,SOD、CAT和GSH-Px酶活性显著升高（P〈0.05）;虫源壳聚糖1.0 g/kg和2.0 g/kg补充组与单纯大负荷运动组相比：SOD、CAT和GSH-Px酶活性升高极为显著（P〈0.01）。MDA含量在不同水平虫源壳聚糖补充组均显著低于单纯大负荷运动组（P〈0.01）。对SOD基因表达分析结果显示,SOD酶活性的升高与其基因表达水平提高呈正相关。结论：补充虫源壳聚糖有助于提高训练大鼠的运动肝脏抗氧化酶系活性,活性的提高与相关编码基因的表达水平提高有关。
목적：보도료보충충원각취당대대서간장항양화매계화SOD기인표체영향적연구결과。방법：대대부하유영훈련대서보충제량분별위각조평균체중적0.5 g/kg、1.0 g/kg화2.0 g/kg적충원각취당,재8주대부하훈련결속후측정간장항양화매계적활성,병분석불동처리조동물간장SOD기인표체수평차이。결과：보충충원각취당능구현저제고항양화매계활성,충원각취당0.5 g/kg보충조여단순대부하운동조상비,SOD、CAT화GSH-Px매활성현저승고（P〈0.05）;충원각취당1.0 g/kg화2.0 g/kg보충조여단순대부하운동조상비：SOD、CAT화GSH-Px매활성승고겁위현저（P〈0.01）。MDA함량재불동수평충원각취당보충조균현저저우단순대부하운동조（P〈0.01）。대SOD기인표체분석결과현시,SOD매활성적승고여기기인표체수평제고정정상관。결론：보충충원각취당유조우제고훈련대서적운동간장항양화매계활성,활성적제고여상관편마기인적표체수평제고유관。
The effects of chitosan on antioxidant enzymes and CuSOD gene expression level in rat liver were analyzed in this study.The chitosan were supplemented to rats that were given swimming training at three dose levels 0.5 g/kg,1.0 g/kg and 2.0 g/kg.The activities of antioxidant enzymes and CuSOD gene expression level in rat liver were measured after eight weeks acute exercise.The activities of antioxidant enzymes were all increased among chitosan supplemented groups compared to non-supplemented exercise rats.SOD,CAT and GSH-Px at 0.5 g/kg supplementation level were increased significantly compared to non-supplemented exercise rats（P0.05）.The activities of these three enzymes at 1.0 g/kg and 2.0 g/kg supplementation levels were increased significantly（P0.01） compared to non-supplemented exercise rats.The MDA concentration were much lower than non-supplemented exercise rats（P0.01）.The results shows that the activities of antioxidant enzymes in rat liver were improved significantly by insect chitosan supplementation,and the gene expression level was consistent with the SOD activity increase.It suggests that the mechanism of improvement on antioxidant enzymes activities by insect chitosan was probably an increase result of their encoded genes expression level.