周口师范学院学报
週口師範學院學報
주구사범학원학보
Journal of Zhoukou Normal University
2011年
2期
65~67
,共null页
体积排阻色谱法 抗EGF单克隆抗体 柱效 理论塔板数
體積排阻色譜法 抗EGF單剋隆抗體 柱效 理論塔闆數
체적배조색보법 항EGF단극륭항체 주효 이론탑판수
size exclusion chromatography; anti -EGF monoclonal antibody; column performance;theoretical plate num-ber
探讨了流动相条件及流速对体积排阻色谱分离抗EGF单克隆抗体的影响．结果表明：选择100mmol／L磷酸钠-150mmol／L氯化钠（pH＝7．0）为流动相，设定流速为0．6mL／min时，抗EGF单克隆抗体在体积排阻色谱柱上的分离能获得最佳柱效．
探討瞭流動相條件及流速對體積排阻色譜分離抗EGF單剋隆抗體的影響．結果錶明：選擇100mmol／L燐痠鈉-150mmol／L氯化鈉（pH＝7．0）為流動相，設定流速為0．6mL／min時，抗EGF單剋隆抗體在體積排阻色譜柱上的分離能穫得最佳柱效．
탐토료류동상조건급류속대체적배조색보분리항EGF단극륭항체적영향．결과표명：선택100mmol／L린산납-150mmol／L록화납（pH＝7．0）위류동상，설정류속위0．6mL／min시，항EGF단극륭항체재체적배조색보주상적분리능획득최가주효．
The effect of mobile phase and flow on separation of anti- EGF monoclonal antibody in size exclusion chromatography has been studied. The result showed that anti - EGF monoclonal antibody separated by size exclusion chromatography could have the best column performance in the condition, when selecting 100mM phosphate buffer/150mM sodium chloride （pHT. 0） as mobile phase and setting flow rate 0.6 mL/min.
