北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2011年
5期
43~47
,共null页
魏宏文 矫玮 张有志 张黎明 陈红霞 薛瑞
魏宏文 矯瑋 張有誌 張黎明 陳紅霞 薛瑞
위굉문 교위 장유지 장려명 진홍하 설서
运动 慢性应激抑郁模型 神经营养
運動 慢性應激抑鬱模型 神經營養
운동 만성응격억욱모형 신경영양
depression; exercise; neurotrophy
目的:观察运动对慢性应激抑郁模型大鼠海马神经营养通路的影响,探讨其抗抑郁作用可能的部分机理。方法:56只雄性大鼠随机分为7组:Control组、CUS组、氟西汀组(Flu)、小强度跑台训练(LIR)、中等强度跑台训练(MIR)、Flu+LIR、Flu+MIR。应用免疫组织化学法和western-blotting法,检测运动和氟西汀对CUS大鼠海马神经元形态、结构、数目,海马神经细胞微管相关蛋白-2C(MAP-2C)及神经营养通路重要信号分子细胞外信号调节激酶(ERK)、磷酸化ERK(pERK)、脑源性神经营养因子(BDNF)、VGF神经肽蛋白表达的影响。结果:1)CUS显著下调海马pERK、BDNF和VGF的表达,Flu、MIR、Flu+MIR和Flu+LIR,均能够显著改善这种情况。Flu+LIR显著上调CUS大鼠海马pERK和BDNF的表达,未能明显上调VGF的表达;2)对CUS大鼠海马pERK表达的上调,MIR、Flu+LIR显著优于LIR。对CUS大鼠BDNF表达的上调,Flu+MIR显著优于Flu或者MIR的单独干预;Flu+LIR显著优于LIR的单独干预。结论:1)运动可以上调慢性应激大鼠海马BDNF及其相关的神经营养通路重要信号分子pERK和VGF的表达。这有利于保护应激状态下海马结构和功能的进一步被破坏,促进神经营养和神经可塑性。2)运动能够增强氟西汀的神经营养作用。
目的:觀察運動對慢性應激抑鬱模型大鼠海馬神經營養通路的影響,探討其抗抑鬱作用可能的部分機理。方法:56隻雄性大鼠隨機分為7組:Control組、CUS組、氟西汀組(Flu)、小彊度跑檯訓練(LIR)、中等彊度跑檯訓練(MIR)、Flu+LIR、Flu+MIR。應用免疫組織化學法和western-blotting法,檢測運動和氟西汀對CUS大鼠海馬神經元形態、結構、數目,海馬神經細胞微管相關蛋白-2C(MAP-2C)及神經營養通路重要信號分子細胞外信號調節激酶(ERK)、燐痠化ERK(pERK)、腦源性神經營養因子(BDNF)、VGF神經肽蛋白錶達的影響。結果:1)CUS顯著下調海馬pERK、BDNF和VGF的錶達,Flu、MIR、Flu+MIR和Flu+LIR,均能夠顯著改善這種情況。Flu+LIR顯著上調CUS大鼠海馬pERK和BDNF的錶達,未能明顯上調VGF的錶達;2)對CUS大鼠海馬pERK錶達的上調,MIR、Flu+LIR顯著優于LIR。對CUS大鼠BDNF錶達的上調,Flu+MIR顯著優于Flu或者MIR的單獨榦預;Flu+LIR顯著優于LIR的單獨榦預。結論:1)運動可以上調慢性應激大鼠海馬BDNF及其相關的神經營養通路重要信號分子pERK和VGF的錶達。這有利于保護應激狀態下海馬結構和功能的進一步被破壞,促進神經營養和神經可塑性。2)運動能夠增彊氟西汀的神經營養作用。
목적:관찰운동대만성응격억욱모형대서해마신경영양통로적영향,탐토기항억욱작용가능적부분궤리。방법:56지웅성대서수궤분위7조:Control조、CUS조、불서정조(Flu)、소강도포태훈련(LIR)、중등강도포태훈련(MIR)、Flu+LIR、Flu+MIR。응용면역조직화학법화western-blotting법,검측운동화불서정대CUS대서해마신경원형태、결구、수목,해마신경세포미관상관단백-2C(MAP-2C)급신경영양통로중요신호분자세포외신호조절격매(ERK)、린산화ERK(pERK)、뇌원성신경영양인자(BDNF)、VGF신경태단백표체적영향。결과:1)CUS현저하조해마pERK、BDNF화VGF적표체,Flu、MIR、Flu+MIR화Flu+LIR,균능구현저개선저충정황。Flu+LIR현저상조CUS대서해마pERK화BDNF적표체,미능명현상조VGF적표체;2)대CUS대서해마pERK표체적상조,MIR、Flu+LIR현저우우LIR。대CUS대서BDNF표체적상조,Flu+MIR현저우우Flu혹자MIR적단독간예;Flu+LIR현저우우LIR적단독간예。결론:1)운동가이상조만성응격대서해마BDNF급기상관적신경영양통로중요신호분자pERK화VGF적표체。저유리우보호응격상태하해마결구화공능적진일보피파배,촉진신경영양화신경가소성。2)운동능구증강불서정적신경영양작용。
Objective: To investigate the effect of exercise on neurotrophic signal pathway in the chronically unpredictable stress(CUS) animal model of depression,and discuss partial antidepressant mechanism of exercise.Methods: 56 male SD rats were divided into seven groups: Control,CUS,Fluoxetine(Flu),low intensity treadmill running(LIR),moderate intensity treadmill running(MIR),Flu+LIR and Flu+MIR.After four weeks of CUS,hippocampal brain-derived neurotrophic factor(BDNF),extracellular signal regulated kinase(ERK1/2) and phosphor-extracellular signal regulated kinase(pERK1/2),and neuropeptide VGF expression were tested using western-blot.Results: 1) CUS down-regulated significantly the expression of pERK,BDNF and VGF in hippocampus.Flu,MIR,Flu+LIR and Flu+MIR could improve these changes obviously .Flu+LIR up-reguated the expression of hippocampal pERK and BDNF but did not affect the expression of hippocampal VGF markedly.2) The up-regulaed effect of MIR and Flu+LIR on the expression of hippocampal pERK was better than that of LIR.The up-regulated effect of Flu+MIR on the expression of hippocampal BDNF was better than that of Flu or MIR respectively;Flu+LIR was better than LIR.Conclusions: The antidepressant mechanisms of exercise may be related to following: exercise can antagonize the chronic stress-induced hipocampal impairment via: 1) up-regalting the expression of hippocampal BDNF,pERK and VGF which were related to BDNF's signal pathway may be underlying the neurotrophic and neuroplastic effects of exercise;2) The neurotrophic effect of fluoxetine can be enhanced further by exercise.