北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2011年
5期
55~59
,共null页
杨锐睿 周越 安江红 高红 邵晶 付劲德 周丽丽 伊木清 王启荣
楊銳睿 週越 安江紅 高紅 邵晶 付勁德 週麗麗 伊木清 王啟榮
양예예 주월 안강홍 고홍 소정 부경덕 주려려 이목청 왕계영
延迟性肌肉损伤 离心运动 Trim63 大豆蛋白 海洋肽
延遲性肌肉損傷 離心運動 Trim63 大豆蛋白 海洋肽
연지성기육손상 리심운동 Trim63 대두단백 해양태
delayed onset muscle injuries; eccentric exercise; Trim63; soybean protein; marine bioactive peptide
目的：观察补充大豆蛋白和海洋肽对离心运动后大鼠血清CK-MM含量和骨骼肌中泛素连接酶Trim63mRNA基因表达的变化。方法：雄性SD大鼠72只,分为安静组、单纯运动组、运动＋大豆蛋白组和运动＋海洋肽组,进行六周跑台适应性离心运动（坡度-5°,前三周跑速分别为20、30、32.5 m/min,后三周速度35 m/min,2.5 min×6次,间歇1 min）,最后一次运动后即刻、24 h、48 h取大鼠血清及股中肌备测。结果：补充大豆蛋白和海洋肽使延迟性肌肉损伤大鼠血清CK-MM含量减少（49.79±25.18 vs.52.40±12.86）;运动后48 h单纯运动组Trim63基因表达较同组即刻明显增加（2.75±1.90vs.0.55±0.54,P〈0.01）;运动后48 h运动＋补充大豆蛋白组Trim63基因表达较同时刻单纯运动组明显减少（0.43±0.09 vs.2.75±1.90,P〈0.01）,而且明显高于运动＋海洋肽组（1.16±0.21 vs.0.43±0.09,P〈0.05）。结论：相比海洋肽而言,同剂量大豆蛋白对胞膜的修复作用更好一些;对运动后Trim63基因表达的抑制作用,同剂量大豆蛋白的作用优于海洋肽。
目的：觀察補充大豆蛋白和海洋肽對離心運動後大鼠血清CK-MM含量和骨骼肌中汎素連接酶Trim63mRNA基因錶達的變化。方法：雄性SD大鼠72隻,分為安靜組、單純運動組、運動＋大豆蛋白組和運動＋海洋肽組,進行六週跑檯適應性離心運動（坡度-5°,前三週跑速分彆為20、30、32.5 m/min,後三週速度35 m/min,2.5 min×6次,間歇1 min）,最後一次運動後即刻、24 h、48 h取大鼠血清及股中肌備測。結果：補充大豆蛋白和海洋肽使延遲性肌肉損傷大鼠血清CK-MM含量減少（49.79±25.18 vs.52.40±12.86）;運動後48 h單純運動組Trim63基因錶達較同組即刻明顯增加（2.75±1.90vs.0.55±0.54,P〈0.01）;運動後48 h運動＋補充大豆蛋白組Trim63基因錶達較同時刻單純運動組明顯減少（0.43±0.09 vs.2.75±1.90,P〈0.01）,而且明顯高于運動＋海洋肽組（1.16±0.21 vs.0.43±0.09,P〈0.05）。結論：相比海洋肽而言,同劑量大豆蛋白對胞膜的脩複作用更好一些;對運動後Trim63基因錶達的抑製作用,同劑量大豆蛋白的作用優于海洋肽。
목적：관찰보충대두단백화해양태대리심운동후대서혈청CK-MM함량화골격기중범소련접매Trim63mRNA기인표체적변화。방법：웅성SD대서72지,분위안정조、단순운동조、운동＋대두단백조화운동＋해양태조,진행륙주포태괄응성리심운동（파도-5°,전삼주포속분별위20、30、32.5 m/min,후삼주속도35 m/min,2.5 min×6차,간헐1 min）,최후일차운동후즉각、24 h、48 h취대서혈청급고중기비측。결과：보충대두단백화해양태사연지성기육손상대서혈청CK-MM함량감소（49.79±25.18 vs.52.40±12.86）;운동후48 h단순운동조Trim63기인표체교동조즉각명현증가（2.75±1.90vs.0.55±0.54,P〈0.01）;운동후48 h운동＋보충대두단백조Trim63기인표체교동시각단순운동조명현감소（0.43±0.09 vs.2.75±1.90,P〈0.01）,이차명현고우운동＋해양태조（1.16±0.21 vs.0.43±0.09,P〈0.05）。결론：상비해양태이언,동제량대두단백대포막적수복작용경호일사;대운동후Trim63기인표체적억제작용,동제량대두단백적작용우우해양태。
Purpose： The purpose of this study was to investigate the ability of soybean protein and marine bioactive peptide to ameliorate exercise-induced injury following eccentric exercise.Methods： 72 Male Sprague-Dawley rats were randomly divided into 3 groups to receive 6 weeks of training before acute about of eccentric exercise in order to set up athlete model training with supplementation of supplying marine bioactive peptide,soybean protein and water,respectively.Exercise training consisted of downhill interval running 5 days /wk for 6 wk（-5°,20,30,32.5 m/min for first 3 wk,respectively and 35 m/min for the last 3 wk,6 bouts of 2.5min running with 1min resting interval）.Vastus intermedius were collected separately at 0h,24h and 48h following last treadmill training.Trim63 mRNA expression in skeletal muscle was determined by QRT-PCR.Serum CK-MM was detected by HYDRASYS Electrophoresis Instrument.Results： Serum CK-MM in group supplying soybean protein was reduced than group with water at 48h（49.79±25.18 vs.52.40±12.86） but no significant different.Trim63 mRNA level in group supplying soybean protein was significantly lower than group with water （0.43±0.09 vs.2.75±1.90,P0.01） at 48h post acute eccentric exercise.However,no significant difference of Trim63 mRNA expression was found between group supplying marine bioactive peptide and group with water.There was significant different between groups supplying soybean protein and marine bioactive peptide in regarding to Trim63 mRNA expression at 48h post acute eccentric exercise（1.16 ±0.21 vs.0.43 ±0.09,P0.05）.Conclusions： Soybean protein supplementation can partially inhibit Trim63 mRNA expression.It suggests soybean protein has more benefits than marine bioactive peptide on ameliorating.
