西藏大学学报
西藏大學學報
서장대학학보
Journal of Tibet University
2011年
2期
7~14
,共null页
段双全 陈彬 张正果 央宗 久美 铁馥荔
段雙全 陳彬 張正果 央宗 久美 鐵馥荔
단쌍전 진빈 장정과 앙종 구미 철복려
虫草属 分生孢子 生物学特性 系统发育分析 侵染机制
蟲草屬 分生孢子 生物學特性 繫統髮育分析 侵染機製
충초속 분생포자 생물학특성 계통발육분석 침염궤제
Cordyceps; Conidiophore; Biological characteristics; Phylogenetic analysis; Infecting machenism
研究从新鲜冬虫夏草分离得到的菌株的形态学特征,并验证其对蝙蝠蛾幼虫的侵染能力。对菌株分化的两个亚型菌株进行了生物学特性研究,并对rRNA基因ITS片段进行了系统发育分析。结果表明:①菌株在18±4℃条件下培养两周后,产生乳白色突起,里面有淡黄色孢子,这种情况下的菌体在侵染蝙蝠蛾幼虫后,20天后长出了子实体;②菌株进一步分化成无性型的两个亚型菌株CC-1和CC-3,这两株菌在表观上差异不明显,但是其分生孢子形态上有显著差异。菌株CC-1的分生孢子为线型孢子,而CC-3的分生孢子却为镰刀型孢子,两菌株均存在有性生殖和无性生殖两种生殖方式;③对无性型两菌株CC-1和CC-3的rRNA基因ITS片段的系统发育分析表明,两株菌均与冬虫夏草非常相似。
研究從新鮮鼕蟲夏草分離得到的菌株的形態學特徵,併驗證其對蝙蝠蛾幼蟲的侵染能力。對菌株分化的兩箇亞型菌株進行瞭生物學特性研究,併對rRNA基因ITS片段進行瞭繫統髮育分析。結果錶明:①菌株在18±4℃條件下培養兩週後,產生乳白色突起,裏麵有淡黃色孢子,這種情況下的菌體在侵染蝙蝠蛾幼蟲後,20天後長齣瞭子實體;②菌株進一步分化成無性型的兩箇亞型菌株CC-1和CC-3,這兩株菌在錶觀上差異不明顯,但是其分生孢子形態上有顯著差異。菌株CC-1的分生孢子為線型孢子,而CC-3的分生孢子卻為鐮刀型孢子,兩菌株均存在有性生殖和無性生殖兩種生殖方式;③對無性型兩菌株CC-1和CC-3的rRNA基因ITS片段的繫統髮育分析錶明,兩株菌均與鼕蟲夏草非常相似。
연구종신선동충하초분리득도적균주적형태학특정,병험증기대편복아유충적침염능력。대균주분화적량개아형균주진행료생물학특성연구,병대rRNA기인ITS편단진행료계통발육분석。결과표명:①균주재18±4℃조건하배양량주후,산생유백색돌기,리면유담황색포자,저충정황하적균체재침염편복아유충후,20천후장출료자실체;②균주진일보분화성무성형적량개아형균주CC-1화CC-3,저량주균재표관상차이불명현,단시기분생포자형태상유현저차이。균주CC-1적분생포자위선형포자,이CC-3적분생포자각위렴도형포자,량균주균존재유성생식화무성생식량충생식방식;③대무성형량균주CC-1화CC-3적rRNA기인ITS편단적계통발육분석표명,량주균균여동충하초비상상사。
To study morphological characteristics of the separated strain from fresh Cordyceps sinensis was studied to validate the infecting effectiveness of the strain on larva of Hepianlus. The differentiation on biological characteristics of two substrains was investigated, and phylogenetic analysis was conducted for ITS spacer of rRNA gene. The results show that: (1) Some white apophysis grew on the strain with shallow yellow spores in it when it was incubated under the condition of 18+4℃for two weeks, and under such condition the larvae of hepianlus were incubated with the strain and the fruiting body growing from the body of larve in 20 days; (2) the strain was separated continually into two substrains, i.e. CC-1 and strain CC-3 and this two substrains were not very different apparently, but there is notable differences on morphology of conidiophores between two substrains, strain CC-1 with line-type conidiophores and strain CC-3 with falciform conidiophores and both substrains having with sexual and asexual reproductions; (3) there is a similarity on ITS spacer sequences of rRNA gene between two anamorphic substrains and that of cordyceps sinensis.